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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||47 Antibodies||182 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies||17 Antibodies|
|Immunofluorescence (IF)||28 Antibodies||59 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||158 Antibodies|
|Immunohistochemistry (IHC)||75 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||54 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||153 Antibodies|
|Dot Blot (DB)||45 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Antigen||C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This ABL1 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|7 references available|
|Supplier||Log in to see|
Product Details anti-ABL1 AntibodyTarget Details ABL1 Application Details Handling References for anti-ABL1 Antibody (ABIN967410) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Recombinant Mouse Abl Gag Fusion Protein|
Target Details ABL1Product Details anti-ABL1 Antibody Application Details Handling References for anti-ABL1 Antibody (ABIN967410) Images back to top
|Alternative Name||Abl (ABL1 Antibody Abstract)|
|Background||The proto-oncogene c-abl was first isolated from the mouse genome as a gene with similarity to the v-abl oncogene of Abelson murine leukemia virus. The c-abl gene encodes a protein tyrosine kinase that is localized in the cytoplasm and nucleus. The c-abl protein shares several common features with other cytoplasmic tyrosine kinases, including the src-homology domains 2 (SH2) and 3 (SH3). The SH2 domain is believed to bind specifically to tyrosine residues of other proteins. The function of the SH3 domain is still unclear. Unique to the c-abl tyrosine kinase is a large C-terminal segment which seems to be essential for its biological function, since mice homozygous for a C-terminal deletion of c-abl have multiple defects at birth. The C-terminal fragment of c-abl contains a DNA-binding domain, and the DNA-binding affinity of this domain seems to be regulated by phosphorylation of critical serine/threonine residues. The c-abl proto-oncogene can be activated in a variety of ways. For example, in Philadelphia chromosome (Ph1)-positive leukemias the c-abl proto-oncogene on chromosome 9 becomes fused to the bcr gene on chromosome 22, and bcr-abl fusion proteins are produced. Ph1-positive cells express either the a-typical 210 kDa bcr-abl fusion protein or a smaller 185 kDa bcr-abl fusion protein. The bcr sequences activate the c-abl tyrosine kinase by deregulating its expression, and actin filament-binding function associated with c-abl is also activated. Expression of bcr-abl fusion proteins in vitro leads to transformation of pre-B lymphoid cells supporting their role as an oncogene. The phosphorylated form of c-abl is observed at ~145 kDa on SDS/PAGE. The 8E9 clone has been reported to react with an epitope in the tyrosine kinase domain of murine abl proteins [Wang et al.]. This antibody is routinely tested by western blot analysis.|
|Molecular Weight||145 kDa|
Application DetailsProduct Details anti-ABL1 Antibody Target Details ABL1 Handling References for anti-ABL1 Antibody (ABIN967410) Images back to top
Related Products: ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-ABL1 Antibody Target Details ABL1 Application Details References for anti-ABL1 Antibody (ABIN967410) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at 4° C.|
References for anti-ABL1 Antibody (ABIN967410)Product Details anti-ABL1 Antibody Target Details ABL1 Application Details Handling Images back to top
|Product cited in:||
Guo, Hirsch-Ginsberg, Xian et al.: "Acute lymphoid leukemia molecular phenotype in a patient with benign-phase chronic myelogenous leukemia." in: Hematologic pathology, Vol. 7, Issue 2, pp. 91-106, 1993 (PubMed).
Guo, Lian, Xian et al.: "BCR-ABL protein expression in peripheral blood cells of chronic myelogenous leukemia patients undergoing therapy." in: Blood, Vol. 83, Issue 12, pp. 3629-37, 1994 (PubMed).
Wang: "Negative regulation of c-abl tyrosine kinase by its variable N-terminal amino acids." in: Oncogene research, Vol. 3, Issue 3, pp. 293-8, 1989 (PubMed).
Kipreos, Lee, Wang: "Isolation of temperature-sensitive tyrosine kinase mutants of v-abl oncogene by screening with antibodies for phosphotyrosine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 5, pp. 1345-9, 1987 (PubMed).
Guo, Wang, Arlinghaus: "Detection of BCR-ABL proteins in blood cells of benign phase chronic myelogenous leukemia patients." in: Cancer research, Vol. 51, Issue 11, pp. 3048-51, 1991 (PubMed).
McWhirter, Wang: "An actin-binding function contributes to transformation by the Bcr-Abl oncoprotein of Philadelphia chromosome-positive human leukemias." in: The EMBO journal, Vol. 12, Issue 4, pp. 1533-46, 1993 (PubMed).
McWhirter, Wang: "Activation of tyrosinase kinase and microfilament-binding functions of c-abl by bcr sequences in bcr/abl fusion proteins." in: Molecular and cellular biology, Vol. 11, Issue 3, pp. 1553-65, 1991 (PubMed).
ImagesProduct Details anti-ABL1 Antibody Target Details ABL1 Application Details Handling References for anti-ABL1 Antibody (ABIN967410) back to top