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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||17 Antibodies||37 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies||8 Antibodies|
|Immunofluorescence (IF)||7 Antibodies||6 Antibodies|
|Immunohistochemistry (IHC)||19 Antibodies||20 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||11 Antibodies||10 Antibodies|
|Immunoprecipitation (IP)||7 Antibodies|
|Simple Western (SimWes)||1 Antibodies|
|Western Blotting (WB)||39 Antibodies|
|Flow Cytometry (FACS)||20 Antibodies|
|Antigen||Annexin A5 (ANXA5) Antibodies|
|Epitope||AA 2-320 Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Annexin V antibody is un-conjugated Alternatives|
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Annexin V AntibodyTarget Details Annexin V Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Annexin A5(ANXA5) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: annexin A5
Protein Name: Annexin A5
|Purification||Immunogen affinity purified.|
|Immunogen||E.coli-derived human Annexin V recombinant protein (Position: A2-D320). Human Annexin V shares 94% and 92% amino acid (aa) sequences identity with mouse and rat Annexin V, respectively.|
Target Details Annexin VProduct Details anti-Annexin V Antibody Application Details Handling Images back to top
|Alternative Name||ANXA5 (ANXA5 Antibody Abstract)|
|Background||Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.|
Application DetailsProduct Details anti-Annexin V Antibody Target Details Annexin V Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for Annexin V is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Annexin V Antibody Target Details Annexin V Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-Annexin V Antibody Target Details Annexin V Application Details Handling back to top