Human APOC1 ELISA Kit

Recommended APOC1 Product (supplied by: Log in to see )

Antigen
Apolipoprotein C-I (APOC1) ELISA Kits
  • APOC1
  • apoc1
  • ALPCI
  • Apo-CIB
  • ApoC-IB
  • LRRG04
  • apolipoprotein C-I
  • Apolipoprotein C-I
  • APOC1
  • apoc1
  • Apoc1
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
21
12
8
7
3
3
2
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
3-50000 pg/mL
Minimum Detection Limit
3 pg/mL
Application
ELISA
Options
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Catalog No. ABIN1979484
$ 666.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN1028132 Sandwich ELISA Biological Fluids, Plasma, Serum 4.687-300 ng/mL 4.687 ng/mL Log in to see
10.090268 ABIN612666 Sandwich ELISA Plasma, Cell Culture Supernatant 0.06 µg/mL Log in to see 12
10.090268 ABIN828055 Sandwich ELISA Serum, Plasma 0.312-20 μg/mL 0.312 μg/mL Log in to see
10.090268 ABIN414953 Sandwich ELISA Serum, Plasma, Biological Fluids 4.69-300 ng/mL 4.69 ng/mL Log in to see
10.090268 ABIN2345031 Sandwich ELISA Biological Fluids, Serum, Plasma Log in to see 1
10.090268 ABIN1113528 Sandwich ELISA Serum, Plasma, Biological Fluids 9.375-600 ng/mL 9.375 ng/mL Log in to see
1 ABIN5563432 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 3.2-50000 pg/mL 3.2 pg/mL Log in to see
1 ABIN5564557 Sandwich ELISA Cell Culture Cells, Plasma, Serum 0.015-1 μg/mL 0.015 μg/mL Log in to see
1 ABIN511214 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 100-2500 ng/mL 100 ng/mL Log in to see
1 ABIN5591560 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 1.56-100 ng/mL 1.56 ng/mL Log in to see
1 ABIN5651926 Sandwich ELISA Biological Fluids, Plasma, Serum 4.69 ng/mL - 300 ng/mL 4.69 ng/mL Log in to see
1 ABIN2683476 Sandwich ELISA Cell Culture Cells, Cell Lysate, Plasma, Serum 0.063-4 μg/mL 0.063 μg/mL Log in to see 3
1 ABIN5519745 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 9.375-600 ng/mL 9.375 ng/mL Log in to see
1 ABIN2541632 Sandwich ELISA Serum, Plasma, Biological Fluids 9.38-600 pg/mL 9.38 pg/mL Log in to see
1 ABIN4967257 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 4.687-300 ng/mL 4.687 ng/mL Log in to see
1 ABIN5596611 Sandwich ELISA Log in to see
1 ABIN2945344 9.375-600 ng/mL 9.375 ng/mL Log in to see
1 ABIN454428 1.56-100 ng/mL 1.56 ng/mL Log in to see
1 ABIN780309 Log in to see
1 ABIN5073399 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

General

Antigen Apolipoprotein C-I (APOC1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(21), (12), (8), (7), (3), (3), (2), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 3-50000 pg/mL
Minimum Detection Limit 3 pg/mL
Application ELISA
Supplier Log in to see

Product Details APOC1 ELISA Kit

Target details Application Details Handling Images
Purpose Human ApoC1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA antibody pair detects human Apo C1. Other species not determined.
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details APOC1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Apo C1 (APOC1 ELISA Kit Abstract)
Gene ID 341
UniProt P02654
Pathways Apoptosis

Application Details

Product Details APOC1 ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples100,000 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Reagent Preparation 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. 2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 100,000 fold*. The Human Apo C1 ELISA Kit Protocol 3 For example, add 1 µL of serum/plasma into a tube with 249 µL Assay Diluent A to prepare a 250-fold diluted sample. Mix through and then pipette 1 µL of prepared 250-fold diluted sample into a tube with 399 µL 1x Assay Diluent A to prepare a final 100,000 fold diluted sample. * Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator. 3. Assay Diluent B should be diluted 5-fold with deionized or distilled water before use. 4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture supernates/urine) into Item C vial to prepare a 50,000 pg/ml standard. Dissolve the powder thoroughly by a gentle mix. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the 50,000 pg/ml standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/ml). Standard, Item C + 400 µL 100 µL 100 µL 100 µL 100 µL 100 µL 100myl 50,000 10,000 2,000 400 80 16 3.2 0 pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml pg/ml 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 mL of The Human Apo C1 ELISA Kit Protocol 4 Wash Buffer Concentrate into deionized or distilled water to yield 400 mL of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 3,000-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 4 µL of HRP-Streptavidin concentrate into a tube with 12 mL 1x Assay Diluent B to prepare a 3,000-fold diluted HRP- Streptavidin solution (don't store the diluted solution for next day use). Mix well.
Assay Procedure 1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or overnight at 4 °C with gentle shaking. 3. Discard essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
Calculation of Results Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
Assay Precision Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only

Handling

Product Details APOC1 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details APOC1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for Apolipoprotein C-I (APOC1) ELISA Kit (ABIN1979484) Apolipoprotein C-I (APOC1) ELISA Kit