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anti-Human BAK1 antibody for Enzyme Immunoassay

Recommended BAK1 Antibody (supplied by: Log in to see )

Antigen
BCL2-Antagonist/killer 1 (BAK1) Antibodies
  • ATBAK1
  • ATSERK3
  • BAK
  • Bak
  • BAK-LIKE
  • Bak1
  • BCL2L7
  • BRI1-associated receptor kinase
  • CDN1
  • ELG
  • ELONGATED
  • F17M5.190
  • F17M5_190
  • N-Bak
  • N-BAK1
  • RECEPTOR KINASES LIKE SERK 10
  • RKS10
  • SERK3
  • SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3
Epitope
BH3 Domain, N-Term
75
20
13
10
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
185
73
34
4
4
1
1
1
1
Host
Rabbit
136
28
22
1
Clonality
Polyclonal
Conjugate
This BAK1 antibody is un-conjugated
4
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
157
72
60
31
31
29
13
11
4
3
3
2
Supplier
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Catalog No. ABIN356810
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN492384 EIA IF WB Mouse IgG2a AA 29-187 Log in to see AT8B4 3
0.0008867729 ABIN499405 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.0008867729 ABIN782900 EIA WB Goat N-Term Log in to see Polyclonal
0.0008867729 ABIN374174 EIA WB Goat N-Term Log in to see Polyclonal

Top referenced anti-BAK1 antibody for Enzyme Immunoassay

Similar anti-BAK1 Antibodies

Application / Reactivity Human
ELISA 74 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 61 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 33 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Western Blotting (WB) 160 Antibodies

General

Antigen BCL2-Antagonist/killer 1 (BAK1) Antibodies
Epitope BH3 Domain, N-Term
(75), (20), (13), (10), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(185), (73), (34), (4), (4), (1), (1), (1), (1)
Host Rabbit
(136), (28), (22), (1)
Clonality
Polyclonal
Conjugate This BAK1 antibody is un-conjugated
(4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(157), (72), (60), (31), (31), (29), (13), (11), (4), (3), (3), (2)
Pubmed 5 references available
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Product Details anti-BAK1 Antibody

Target Details BAK1 Application Details Handling References for anti-BAK1 Antibody (ABIN356810) Images
Specificity This antibody is specific to BAK (BH3 Domain).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human Bak.
Isotype Ig

Target Details BAK1

Product Details anti-BAK1 Antibody Application Details Handling References for anti-BAK1 Antibody (ABIN356810) Images back to top
Antigen
Alternative Name Bcl-2-Like 7 (BAK1 Antibody Abstract)
Background BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.Synonyms: Apoptosis regulator BAK, BAK, BAK1, BCL2L7, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, Bcl2-L-7, CDN1, cell death inhibitor 1
Gene ID 578, 9606
UniProt Q16611
Pathways Apoptosis

Application Details

Product Details anti-BAK1 Antibody Target Details BAK1 Handling References for anti-BAK1 Antibody (ABIN356810) Images back to top
Application Notes ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details References for anti-BAK1 Antibody (ABIN356810) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-BAK1 Antibody (ABIN356810)

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling Images back to top
Background publications

Cartron, Juin, Oliver, Martin, Meflah, Vallette: "Nonredundant role of Bax and Bak in Bid-mediated apoptosis." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4701-12, 2003

Mikhailov, Mikhailova, Degenhardt, Venkatachalam, White, Saikumar: "Association of Bax and Bak homo-oligomers in mitochondria. Bax requirement for Bak reorganization and cytochrome c release." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 5367-76, 2003

Werner, de Vries, Tait, Bontjer, Borst: "Bcl-2 family member Bfl-1/A1 sequesters truncated bid to inhibit is collaboration with pro-apoptotic Bak or Bax." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22781-8, 2002

Bellosillo, Villamor, López-Guillermo, Marcé, Bosch, Campo, Montserrat, Colomer: "Spontaneous and drug-induced apoptosis is mediated by conformational changes of Bax and Bak in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 100, Issue 5, pp. 1810-6, 2002

Grutkoski, Graeber, Ayala, Simms: "Paracrine suppression of apoptosis by cytokine-stimulated neutrophils involves divergent regulation of NF-kappaB, Bcl-X(L), and Bak." in: Shock (Augusta, Ga.), Vol. 17, Issue 1, pp. 47-54, 2002

Images

Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling References for anti-BAK1 Antibody (ABIN356810) back to top
Supplier Images
 image for anti-BAK1 antibody (BCL2-Antagonist/killer 1) (BH3 Domain) (ABIN356810) anti-BCL2-Antagonist/killer 1 (BAK1) (BH3 Domain), (N-Term) antibody
 image for anti-BAK1 antibody (BCL2-Antagonist/killer 1) (BH3 Domain) (ABIN356810) anti-BCL2-Antagonist/killer 1 (BAK1) (BH3 Domain), (N-Term) antibody (Image 2)