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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies|
|Immunofluorescence (IF)||15 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||59 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||31 Antibodies|
|Immunoprecipitation (IP)||14 Antibodies|
|Western Blotting (WB)||141 Antibodies|
|Antigen||BCL2-Antagonist/killer 1 (BAK1) Antibodies|
|Epitope||BH3 Domain, N-Term Alternatives|
|Conjugate||This BAK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-BAK1 AntibodyTarget Details BAK1 Application Details Handling References for anti-BAK1 Antibody (ABIN356810) Images
|Specificity||This antibody is specific to BAK (BH3 Domain).|
|Purification||Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region (BH3 domain) of human Bak.|
Target Details BAK1Product Details anti-BAK1 Antibody Application Details Handling References for anti-BAK1 Antibody (ABIN356810) Images back to top
|Alternative Name||Bcl-2-Like 7 (BAK1 Antibody Abstract)|
|Background||BAK belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BAK localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.Synonyms: Apoptosis regulator BAK, BAK, BAK1, BCL2L7, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, Bcl2-L-7, CDN1, cell death inhibitor 1|
|Gene ID||578, 9606|
Application DetailsProduct Details anti-BAK1 Antibody Target Details BAK1 Handling References for anti-BAK1 Antibody (ABIN356810) Images back to top
ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-BAK1 Antibody Target Details BAK1 Application Details References for anti-BAK1 Antibody (ABIN356810) Images back to top
|Buffer||PBS containing 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-BAK1 Antibody (ABIN356810)Product Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling Images back to top
Cartron, Juin, Oliver et al.: "Nonredundant role of Bax and Bak in Bid-mediated apoptosis." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4701-12, 2003 (PubMed).
Mikhailov, Mikhailova, Degenhardt et al.: "Association of Bax and Bak homo-oligomers in mitochondria. Bax requirement for Bak reorganization and cytochrome c release." in: The Journal of biological chemistry, Vol. 278, Issue 7, pp. 5367-76, 2003 (PubMed).
Bellosillo, Villamor, López-Guillermo et al.: "Spontaneous and drug-induced apoptosis is mediated by conformational changes of Bax and Bak in B-cell chronic lymphocytic leukemia." in: Blood, Vol. 100, Issue 5, pp. 1810-6, 2002 (PubMed).
Werner, de Vries, Tait et al.: "Bcl-2 family member Bfl-1/A1 sequesters truncated bid to inhibit is collaboration with pro-apoptotic Bak or Bax." in: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22781-8, 2002 (PubMed).
Grutkoski, Graeber, Ayala et al.: "Paracrine suppression of apoptosis by cytokine-stimulated neutrophils involves divergent regulation of NF-kappaB, Bcl-X(L), and Bak." in: Shock (Augusta, Ga.), Vol. 17, Issue 1, pp. 47-54, 2002 (PubMed).
ImagesProduct Details anti-BAK1 Antibody Target Details BAK1 Application Details Handling References for anti-BAK1 Antibody (ABIN356810) back to top