anti-Human Caspase 3 antibody for Flow Cytometry

Recommended Caspase 3 Antibody (supplied by: Log in to see )

Antigen
Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Epitope
AA 219-248, C-Term
43
33
27
24
22
21
19
19
16
15
12
12
12
11
10
10
9
7
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
574
307
254
47
46
37
29
27
24
20
18
17
17
12
9
8
7
4
2
2
Host
Rabbit
465
162
19
4
2
Clonality
Polyclonal
Conjugate
This Caspase 3 antibody is un-conjugated
27
24
19
14
9
9
9
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
489
241
219
144
94
76
55
52
45
29
28
13
9
9
7
5
4
3
2
2
2
2
2
2
2
1
1
1
1
Options
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Catalog No. ABIN655271
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.326701 ABIN391813 FACS IHC (p) WB Rabbit Ig AA 60-90, Center Log in to see Polyclonal 3
14.326701 ABIN4897861 FACS Alexa Fluor 488 Rabbit IgG Log in to see 269518
14.326701 ABIN3030305 FACS IHC ELISA WB Rabbit Ig Fraction AA 219-248 Log in to see Polyclonal
14.326701 ABIN3030306 FACS IHC ELISA WB Rabbit Ig Fraction AA 60-90 Log in to see Polyclonal
14.326701 ABIN3030296 FACS IHC ELISA WB Rabbit Ig Fraction AA 149-179 Log in to see Polyclonal
14.326701 ABIN5542610 ELISA FACS ICC IHC WB Mouse IgG1 AA 29-175 Log in to see 3D4D7
1 ABIN252651 CyTOF EM FACS ICC IF IHC IHC (fro) IHC (p) SimWes WB Mouse IgG1 kappa Log in to see 31A1067 107
1 ABIN651176 FACS IHC (p) WB Rabbit Ig AA 149-179, Asp175 Log in to see Polyclonal 1
1 ABIN5556200 EIA FACS IHC (p) WB Rabbit Ig Fraction AA 226-256, C-Term Log in to see Polyclonal
1 ABIN4288095 FACS IHC IHC (p) IP WB Rabbit Log in to see Polyclonal 15
1 ABIN4288106 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 kappa Log in to see 31A1067 1
1 ABIN2665464 FACS Rabbit Log in to see Polyclonal 4
1 ABIN5027753 FACS IHC WB Mouse IgG1 Log in to see ABM11D3
1 ABIN4880596 ELISA FACS WB Mouse IgG1 AA 29-175 Log in to see 3D4D7
1 ABIN4288121 CyTOF ELISA FACS ICC IHC WB Mouse Log in to see 3D4D10
1 ABIN4288122 CyTOF ELISA FACS ICC IHC WB Mouse Log in to see 3D4D7
1 ABIN470500 ICC FACS IF ELISA Alexa Fluor 488 Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2219054 IC FACS IF ELISA Alexa Fluor 488 Rabbit IgG cleaved Log in to see Polyclonal
1 ABIN219229 FACS IHC (fro) IF IHC (p) IP WB Rabbit IgG N-Term Log in to see
1 ABIN2690044 FACS PE Rabbit IgG AA 60-100 Log in to see Polyclonal

General

Antigen Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) Antibodies
Epitope AA 219-248, C-Term
(43), (33), (27), (24), (22), (21), (19), (19), (16), (15), (12), (12), (12), (11), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(574), (307), (254), (47), (46), (37), (29), (27), (24), (20), (18), (17), (17), (12), (9), (8), (7), (4), (2), (2)
Host Rabbit
(465), (162), (19), (4), (2)
Clonality (Clone)
Polyclonal   ( )
Conjugate This Caspase 3 antibody is un-conjugated
(27), (24), (19), (14), (9), (9), (9), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(489), (241), (219), (144), (94), (76), (55), (52), (45), (29), (28), (13), (9), (9), (7), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Caspase 3 Antibody

Target Details Caspase 3 Application Details Handling ProductDetails: References for anti-Caspase 3 antibody (ABIN655271) Images
Specificity This CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-256 amino acids from the C-terminal region of human CASP3.
Predicted Reactivity Mouse (Murine),Monkey,Rat (Rattus),Rabbit
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This CASP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219-248 AA from the C-terminal region of human CASP3.
Clone RB14883
Isotype Ig

Target Details Caspase 3

Product Details anti-Caspase 3 Antibody Application Details Handling ProductDetails: References for anti-Caspase 3 antibody (ABIN655271) Images back to top
Antigen
Alternative Name CASP3 (CASP3 Antibody Abstract)
Background This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].
Synonyms: Caspase-3,CASP3,CPP32
Molecular Weight 31608 DA
Gene ID 836
NCBI Accession NP_004337, NP_116786
UniProt P42574
Research Area Apoptosis/Necrosis, Signaling, Phospho-specific antibodies, Protein Modifications, Metabolism, Cell Structure
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation

Application Details

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Handling ProductDetails: References for anti-Caspase 3 antibody (ABIN655271) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details ProductDetails: References for anti-Caspase 3 antibody (ABIN655271) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-Caspase 3 antibody (ABIN655271)

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling Images back to top
Background publications

Würstle, Laussmann, Rehm: "The caspase-8 dimerization/dissociation balance is a highly potent regulator of caspase-8, -3, -6 signaling." in: The Journal of biological chemistry, Vol. 285, Issue 43, pp. 33209-18, 2010

Srikanth, Wall, Maldonado-Contreras, Shi, Zhou, Demma, Mumy, McCormick: "Salmonella pathogenesis and processing of secreted effectors by caspase-3." in: Science (New York, N.Y.), Vol. 330, Issue 6002, pp. 390-3, 2010

Burke, Smith, Smith: "cIAP1 cooperatively inhibits procaspase-3 activation by the caspase-9 apoptosome." in: The Journal of biological chemistry, Vol. 285, Issue 39, pp. 30061-8, 2010

Images

Product Details anti-Caspase 3 Antibody Target Details Caspase 3 Application Details Handling ProductDetails: References for anti-Caspase 3 antibody (ABIN655271) back to top
Supplier Images
Western Blotting (WB) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 219-248), (C-Term) antibody (ABIN655271) CASP3 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/l...
Immunohistochemistry (IHC) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 219-248), (C-Term) antibody (ABIN655271) CASP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin ...
Flow Cytometry (FACS) image for anti-Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (AA 219-248), (C-Term) antibody (ABIN655271) CASP3 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram...