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anti-Human DFFB antibody for Western Blotting

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • 40kDa
  • 5730477D02Rik
  • CAD
  • Cad
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Didff
  • LOC100223514
Epitope
Middle Region
27
18
13
9
8
5
5
5
4
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
101
69
49
1
Host
Rabbit
125
10
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
106
38
25
22
21
18
17
5
4
3
3
1
Supplier
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Catalog No. ABIN2784747
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.123037994 ABIN951896 FACS IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
0.123037994 ABIN653798 FACS IHC (p) WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 1
0.123037994 ABIN499738 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 5
0.123037994 ABIN499740 EIA IF WB Rabbit IgG Intermediate Domain Log in to see Polyclonal 5
0.123037994 ABIN499736 EIA IF WB Rabbit IgG Log in to see Polyclonal 5
0.123037994 ABIN967293 WB Rabbit Ig Fraction AA 203-218 Log in to see Polyclonal 4
0.123037994 ABIN2707475 WB Rabbit N-Term Log in to see Polyclonal
0.123037994 ABIN266063 WB Mouse IgG Log in to see 36A349
0.123037994 ABIN1858217 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 87-323 Log in to see Polyclonal
0.123037994 ABIN634501 WB Rabbit Log in to see Polyclonal
0.123037994 ABIN2156248 ICC ELISA WB Rabbit AA 3-18 Log in to see Polyclonal
0.123037994 ABIN560596 ELISA WB Mouse AA 229-338, partial Log in to see Polyclonal
0.123037994 ABIN2156250 ICC ELISA WB Rabbit Log in to see Polyclonal
0.123037994 ABIN2156249 ICC WB Rabbit AA 203-218 Log in to see Polyclonal
0.123037994 ABIN2158535 IHC WB Rabbit Ig Fraction Log in to see Polyclonal
0.123037994 ABIN514957 WB Rabbit AA 1-338, full length Log in to see Polyclonal
0.123037994 ABIN1030918 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.123037994 ABIN1031351 ICC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.123037994 ABIN1030919 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.123037994 ABIN189899 WB Rabbit AA 203-218 Log in to see Polyclonal

Top referenced anti-DFFB antibody for Western Blotting

Similar anti-DFFB Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 35 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies
Western Blotting (WB) 84 Antibodies

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope Middle Region
(27), (18), (13), (9), (8), (5), (5), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(101), (69), (49), (1)
Host Rabbit
(125), (10)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(106), (38), (25), (22), (21), (18), (17), (5), (4), (3), (3), (1)
Pubmed 1 reference available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN2784747) Images
Blocking Peptide Blocking peptide for this product available: ABIN975736
Sequence EYFYGLLFTS ENLKLVHIVC HKKTTHKLNC DPSRIYKPQT RLKRKQPVRK
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against DFFB. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human DFFB

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB Antibody (ABIN2784747) Images back to top
Antigen
Alternative Name DFFB (DFFB Antibody Abstract)
Background DFFB is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. DFFB degrades naked DNA and induces apoptotic morphology.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined.
Molecular Weight 39 kDa
Gene ID 1677
NCBI Accession NM_004402, NP_004393
UniProt O76075
Research Area Chromatin and Nuclear Signaling, Cell Cycle, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB Antibody (ABIN2784747) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 338 AA

Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB Antibody (ABIN2784747) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-DFFB Antibody (ABIN2784747)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Neimanis, Albig, Doenecke, Kahle: "Sequence elements in both subunits of the DNA fragmentation factor are essential for its nuclear transport." in: The Journal of biological chemistry, Vol. 282, Issue 49, pp. 35821-30, 2007

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN2784747) back to top
Supplier Images
Western Blotting (WB) image for anti-DFFB antibody (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (Middle Region) (ABIN2784747) WB Suggested Anti-DFFB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...