DFFB antibody (AA 1-289)

Catalog No. ABIN7193449

Product Details anti-DFFB Antibody

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Binding Specificity: AA 1-289

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DFFB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purification: purified

Immunogen: Purified recombinant fragment of human DFFB (AA: 1-289) expressed in E. coli.

Clone: 2E6G3

Isotype: IgG1

Application Details

Application Notes: WB:1:500 - 1:2000, FCM:1:200 - 1:400, ELISA:1:10000,

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: 4°C, -20°C for long term storage

Target Details for DFFB

Target: DFFB (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB))

Alternative Name: DFFB (DFFB Products)

Synonyms: CAD antibody, CPAN antibody, DFF-40 antibody, DFF2 antibody, DFF40 antibody, LOC100223514 antibody, 40kDa antibody, 5730477D02Rik antibody, Didff antibody, Cad antibody, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase antibody, DNA fragmentation factor subunit beta antibody, DNA fragmentation factor, beta polypeptide (caspase-activated DNase) antibody, DNA fragmentation factor, beta subunit antibody, CAD antibody, DFFB antibody, dffb antibody, LOC100223514 antibody, Dffb antibody

Background:

Description: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.

Aliases: CAD, CPAN, DFF2, DFF40, DFF-40

Molecular Weight: 39.1 kDa

Gene ID: 1677

Pathways: Apoptosis, Caspase Cascade in Apoptosis