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anti-Mouse (Murine) DFFB antibody for ELISA

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • 40kDa
  • 5730477D02Rik
  • CAD
  • Cad
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Didff
  • LOC100223514
Epitope
C-Term
28
18
13
9
8
5
5
5
4
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
126
71
52
1
Host
Rabbit
131
28
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
130
41
24
22
22
21
17
8
5
3
3
1
Supplier
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Catalog No. ABIN1001970
$ 455.07
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012092679 ABIN2156250 ICC ELISA WB Rabbit Log in to see Polyclonal
0.0012092679 ABIN1030918 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0012092679 ABIN1030919 ICC ELISA WB Rabbit Middle Region Log in to see Polyclonal
0.0012092679 ABIN1886855 ICC ELISA WB Rabbit AA 203-218 Log in to see Polyclonal
0.0012092679 ABIN1887769 ICC ELISA WB Rabbit Center Log in to see Polyclonal
0.0012092679 ABIN2425633 IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012092679 ABIN2252732 IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.0012092679 ABIN1584351 ICC ELISA WB Rabbit Log in to see Polyclonal
0.0012092679 ABIN1584350 ICC ELISA WB Rabbit Log in to see Polyclonal
0.0012092679 ABIN4305068 ELISA ICC IF WB Rabbit Log in to see Polyclonal
0.0012092679 ABIN4305065 ELISA ICC IF WB Rabbit Log in to see Polyclonal
0.0012092679 ABIN4305067 ELISA ICC IF WB Rabbit Log in to see Polyclonal

Top referenced anti-DFFB antibody for ELISA

Similar anti-DFFB Antibodies

Application / Reactivity Mouse (Murine)
ELISA 13 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunocytochemistry (ICC) 12 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Western Blotting (WB) 51 Antibodies

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope C-Term
(28), (18), (13), (9), (8), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(126), (71), (52), (1)
Host Rabbit
(131), (28)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(130), (41), (24), (22), (22), (21), (17), (8), (5), (3), (3), (1)
Pubmed 4 references available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN1001970) Images
Blocking Peptide Blocking peptide for this product available: ABIN1003762
Purification Affinity chromatography purified via peptide column.
Immunogen Rabbit polyclol CAD antibody was raised against a peptide corresponding to amino acids 314 to 329 of murine CAD .
Isotype IgG

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB Antibody (ABIN1001970) Images back to top
Antigen
Alternative Name CAD (Caspase Activated DNase) (DFFB Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB Antibody (ABIN1001970) Images back to top
Application Notes CAD antibody can be used for detection of CAD by Western blot at 1:500 to 1:1000 dilution. (Optimal dilution should be determined by user.) Tissue lysate from murine lung or kidney can be used as positive control and a 40 kDa band should be detected. Antibody can also be used for immunohistochemistry and ELISA and might be suited for other applications not tested so far. CAD antibody is mouse reactive.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB Antibody (ABIN1001970) Images back to top
Format Liquid
Buffer Antibody is supplied in PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Antibody can be stored at 4 °C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 myl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
Storage 4 °C
Expiry Date 12 months

References for anti-DFFB Antibody (ABIN1001970)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Halenbeck, MacDonald, Roulston, Chen, Conroy, Williams: "CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45." in: Current biology : CB, Vol. 8, Issue 9, pp. 537-40, 1998

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998

Liu, Li, Widlak, Zou, Luo, Garrard, Wang: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998

Liu, Zou, Slaughter, Wang: "DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis." in: Cell, Vol. 89, Issue 2, pp. 175-84, 1997