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|Application / Reactivity||Mouse (Murine)|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies|
|Western Blotting (WB)||48 Antibodies|
|Antigen||DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This DFFB antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-DFFB AntibodyTarget Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN499517) Images
|Specificity||CAD antibody was raised against a peptide corresponding to amino acids 314 to 329 of murine CAD.|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||Human CAD (C-Terminus) Peptide|
Target Details DFFBProduct Details anti-DFFB Antibody Application Details Handling References for anti-DFFB Antibody (ABIN499517) Images back to top
|Alternative Name||DFFB / CAD (DFFB Antibody Abstract)|
|Background||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40 (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei (1-4). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.Synonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB Antibody (ABIN499517) Images back to top
ELISA. Western Blot: CAD antibody antibody can be used for detection of CAD at 1/500 to 1/1000dilution. A 40 kDa band should be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB Antibody (ABIN499517) Images back to top
|Buffer||PBS containing 0.02 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
References for anti-DFFB Antibody (ABIN499517)Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Nagai, Dohmae, Nishimura et al.: "Operando XAFS study of catalytic NO reduction over Cu/CeO2: the effect of copper-ceria interaction under periodic operation." in: Physical chemistry chemical physics : PCCP, Vol. 15, Issue 22, pp. 8461-5, 2013 (PubMed).
Liu, Li, Widlak et al.: "The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 15, pp. 8461-6, 1998 (PubMed).
Halenbeck, MacDonald, Roulston et al.: "CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45." in: Current biology : CB, Vol. 8, Issue 9, pp. 537-40, 1998 (PubMed).
Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998 (PubMed).
Enari, Sakahira, Yokoyama et al.: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998 (PubMed).
ImagesProduct Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB Antibody (ABIN499517) back to top