anti-Mouse (Murine) DFFB antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended DFFB Antibody (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Epitope
AA 205-222
19
18
15
7
5
5
4
4
4
4
3
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
128
76
60
Host
Rabbit
129
29
Clonality
Polyclonal
Conjugate
This DFFB antibody is un-conjugated
6
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
128
38
26
26
21
20
18
10
5
3
1
1
1
Supplier
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Catalog No. ABIN319000
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN724085 IF (p) IHC (p) WB Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN214484 ICC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN5554638 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN724094 IHC (p) WB HRP Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN303254 IF IHC (p) WB Rabbit Center Log in to see Polyclonal
1 ABIN307617 IHC (p) WB Rabbit IgG AA 205-222 Log in to see Polyclonal
1 ABIN5554637 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN724087 IHC (p) WB Biotin Rabbit IgG AA 190-240 Log in to see Polyclonal
1 ABIN1733625 ICC IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN893946 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN893938 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN872776 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) Antibodies
Epitope AA 205-222
(19), (18), (15), (7), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(128), (76), (60)
Host Rabbit
(129), (29)
Clonality
Polyclonal
Conjugate This DFFB antibody is un-conjugated
(6), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(128), (38), (26), (26), (21), (20), (18), (10), (5), (3), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-DFFB Antibody

Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN319000) Images
Specificity This antibody recognises the 40 kDa CAD in Western blots.
Purification Affinity Chromatography
Immunogen Synthetic peptide corresponding to amino acids 205-222 of Mouse CAD
Isotype IgG

Target Details DFFB

Product Details anti-DFFB Antibody Application Details Handling References for anti-DFFB antibody (ABIN319000) Images back to top
Antigen
Alternative Name DFFB / CAD (DFFB Antibody Abstract)
Background CAD (Caspase Activated Deoxyribonuclease) is the murine homologue of human DFF-40. CAD is released and activated by caspase cleavage of ICAD, and eventually causes degradation of nuclear DNA.Synonyms: CPAN, Caspase-activated DNase, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DFF-40, DFF2, DFF40, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor subunit beta
Gene ID 13368
NCBI Accession NP_031885
UniProt O54788
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DFFB Antibody Target Details DFFB Handling References for anti-DFFB antibody (ABIN319000) Images back to top
Application Notes Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DFFB Antibody Target Details DFFB Application Details References for anti-DFFB antibody (ABIN319000) Images back to top
Concentration 0.5 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DFFB antibody (ABIN319000)

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling Images back to top
Background publications

Enari, Sakahira, Yokoyama, Okawa, Iwamatsu, Nagata: "A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD." in: Nature, Vol. 391, Issue 6662, pp. 43-50, 1998

Sakahira, Enari, Nagata: "Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis." in: Nature, Vol. 391, Issue 6662, pp. 96-9, 1998

Images

Product Details anti-DFFB Antibody Target Details DFFB Application Details Handling References for anti-DFFB antibody (ABIN319000) back to top
Supplier Images
 image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 205-222) antibody (ABIN319000) anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 205-222) antibody
 image for anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 205-222) antibody (ABIN319000) anti-DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) (AA 205-222) antibody (Image 2)