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Human DFFB ELISA Kit

Recommended DFFB Product (supplied by: Log in to see )

Antigen
DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) ELISA Kits
  • LOC100223514
  • CAD
  • CPAN
  • DFF-40
  • DFF2
  • DFF40
  • Cad
  • 40kDa
  • 5730477D02Rik
  • Didff
  • DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
  • DNA fragmentation factor, beta subunit
  • DFFB
  • LOC100223514
  • Dffb
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
5
3
2
2
2
2
2
2
1
1
1
Method Type
Sandwich ELISA
Detection Range
0.156-10 ng/mL
Minimum Detection Limit
0.156 ng/mL
Application
ELISA
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Catalog No. ABIN1028321
$ 870.62
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1114001 Sandwich ELISA Serum, Plasma, Biological Fluids 1.563-100 ng/mL 1.563 ng/mL Log in to see
1 ABIN770650 Competition ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 2.5-50 ng/mL 2.5 ng/mL Log in to see
1 ABIN2542546 Sandwich ELISA Tissue Homogenate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN4967809 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN454660 Log in to see

Similar DFFB ELISA Kits

Application / Reactivity Human
ELISA 6 ELISA Kits

General

Antigen DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(5), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 0.156-10 ng/mL
Minimum Detection Limit 0.156 ng/mL
Application ELISA
Supplier Log in to see

Product Details DFFB ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 0.052 ng/mL
Components Assay plate: 1×
Standard: 2×
Sample Diluent : 1 × 20 mL
Assay Diluent A: 1 × 10 mL
Assay Diluent B: 1 × 10 mL
Detection Reagent A: 1 × 120 µL
Detection Reagent B: 1 × 120 µL
Wash Buffer(25 x concentrate): 1 × 30 mL
Substrate: 1 × 10 mL
Stop Solution: 1 × 10 mL
Plate sealer for 96 wells: 5×
Instruction: 1×
Material not included Microplate reader, Pipettes and pipette tips, EP tube, Deionized or distilled water.

Target details

Product Details DFFB ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Caspase Activated DNase (CAD) (DFFB ELISA Kit Abstract)
Background Synonyms: DFF-B, DFFB, CPAN, DFF40, DFF2, DNA Fragmentation Factor 40kDa Beta Polypeptide
Gene ID 1677
UniProt O76075
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details DFFB ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated,Strips (12 x 8)
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Restrictions For Research Use only

Handling

Product Details DFFB ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance. 2. The kit should not be used beyond the expiration date on the kit label. 3. Do not mix or substitute reagents with those from other lots or sources. 4. If samples generate values higher than the highest standard, further dilute the samples with the Sample Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
Storage 4 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months

Images

Product Details DFFB ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for DFFB ELISA Kit (DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase)) (ABIN1028321) DNA Fragmentation Factor, 40kDa, beta Polypeptide (Caspase-Activated DNase) (DFFB) ELISA Kit