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anti-Dog (Canine) DIABLO antibody for Immunofluorescence

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Epitope
AA 125-239
35
32
13
11
8
6
5
4
4
3
3
2
2
2
1
1
1
1
Reactivity
Dog (Canine), Human, Rat (Rattus)
169
62
41
4
3
3
2
1
1
1
Host
Mouse
129
49
9
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
176
78
67
44
30
15
11
9
6
4
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN968785
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Top referenced anti-DIABLO antibody for Immunofluorescence

Similar anti-DIABLO Antibodies

Application / Reactivity Human Rat (Rattus) Dog (Canine)
Western Blotting (WB) 160 Antibodies 42 Antibodies 4 Antibodies
Neutralization (Neut) 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 25 Antibodies 6 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 39 Antibodies 16 Antibodies
Immunohistochemistry (IHC) 57 Antibodies 29 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 125-239
(35), (32), (13), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Rat (Rattus)
(169), (62), (41), (4), (3), (3), (2), (1), (1), (1)
Host Mouse
(129), (49), (9), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(176), (78), (67), (44), (30), (15), (11), (9), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN968785) Images
Cross-Reactivity Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human Smac/DIABLO aa. 125-239
Clone 56-Smac-DIABLO
Isotype IgG2b

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN968785) Images back to top
Antigen
Alternative Name Smac/DIABLO (DIABLO Antibody Abstract)
Background Apoptosis is a genetically programmed, selective process of cell death that occurs during normal cell differentiation and development of multicellular organisms. Inhibitors of apoptosis proteins (IAPs), hILP/XIAP, c-IAP1, c-IAP2, and survivin, suppress apoptosis by preventing procaspase activation and inhibiting mature caspase activity. Smac/DIABLO (second mitochondria-derived activator of caspases) eliminates the inhibitory effect of many IAPs, and has been shown to interact with hILP, c-IAP1, c-IAP2, and survivin. The interaction with hILP occurs through Smac/DIABLO binding to the second and third BIR domains of hILP. Smac/DIABLO protein contains an N-terminal mitochondria targeting sequence (MTS) and a procaspase-3 activation domain. The N-terminal MTS is cleaved after import into the mitochondria, and the activity of the mature Smac/DIABLO is dependent on homodimerization and release from the mitochondria. Overexpression of Smac/DIABLO in HeLa cells increases the sensitivity to UV-mediated apoptosis. Thus, Smac/DIABLO acts as an apoptotic activator through its binding to IAPs and activation of caspases.
Molecular Weight 22 kDa
Research Area Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN968785) Images back to top
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN968785) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-DIABLO Antibody (ABIN968785)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Kim, Broxmeyer: "Therapeutic potential of 4-1BB (CD137) as a regulator for effector CD8(+) T cells." in: Journal of hematotherapy & stem cell research, Vol. 10, Issue 4, pp. 441-9, 2001

Chai, Du, Wu, Kyin, Wang, Shi: "Structural and biochemical basis of apoptotic activation by Smac/DIABLO." in: Nature, Vol. 406, Issue 6798, pp. 855-62, 2000

Du, Fang, Li, Li, Wang: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." in: Cell, Vol. 102, Issue 1, pp. 33-42, 2000

Ikuta, Weissman: "Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 4, pp. 1502-6, 1992

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN968785) back to top
Supplier Images
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (AA 125-239) (ABIN968785) Western blot analysis of Smac/DIABLO on a HeLa cell lysate (Human cervical epitheloid...