anti-Rat (Rattus) DIABLO antibody for Enzyme Immunoassay

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • smac
  • smac3
  • diablo-s
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • Smac
  • DFNA64
  • SMAC
  • DBO
  • XSmac
  • diablo, IAP-binding mitochondrial protein
  • diablo homolog (Drosophila)
  • diablo
  • Diablo
  • DIABLO
Epitope
AA 222-237, C-Term
39
31
12
11
8
6
4
4
4
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
184
70
56
4
4
3
3
3
3
2
1
1
Host
Rabbit
135
53
7
1
Clonality
Polyclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
182
73
66
48
31
20
9
8
7
4
3
3
2
2
1
1
1
1
1
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Catalog No. ABIN5555253
$ 375.38
Plus shipping costs $45.00

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 23 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 55 Antibodies
Flow Cytometry (FACS) 5 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Epitope AA 222-237, C-Term
(39), (31), (12), (11), (8), (6), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(184), (70), (56), (4), (4), (3), (3), (3), (3), (2), (1), (1)
Host Rabbit
(135), (53), (7), (1)
Clonality
Polyclonal
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(182), (73), (66), (48), (31), (20), (9), (8), (7), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling Images
Specificity This antibody detects DIABLO / SMAC at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Immunogen Smac antibody was raised against a peptide corresponding to amino acids 222 to 237 of murine Smac/DIABLO.
Isotype IgG

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling Images back to top
Antigen
Alternative Name DIABLO / SMAC (DIABLO Antibody Abstract)
Background The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1,2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1,2).Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 66593
UniProt Q9JIQ3
Pathways Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling Images back to top
Application Notes ELISA. Western blot: 0.5 to 1 μg/mL, 25 kDa band can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Images back to top
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.