You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) DIABLO antibody for Immunocytochemistry

Recommended DIABLO Antibody (supplied by: Log in to see )

Antigen
Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
  • 0610041G12Rik
  • 1700006L01Rik
  • AU040403
  • DBO
  • DFNA64
  • diablo-s
  • SMAC
  • Smac
  • smac
  • smac3
  • XSmac
Reactivity
Human, Mouse (Murine), Rat (Rattus)
170
61
41
4
4
3
2
1
1
1
Host
Mouse
130
49
9
1
Clonality
Monoclonal
Conjugate
This DIABLO antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
177
78
68
45
29
17
11
10
6
4
2
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN153019
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010083028 ABIN4354645 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 1
0.0010083028 ABIN2974020 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010083028 ABIN4354646 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-DIABLO antibody for Immunocytochemistry

Similar anti-DIABLO Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 17 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 7 Antibodies
Immunofluorescence (IF) 8 Antibodies 9 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 15 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 41 Antibodies
Flow Cytometry (FACS) 6 Antibodies

General

Antigen Second Mitochondria-Derived Activator of Caspase (DIABLO) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(170), (61), (41), (4), (4), (3), (2), (1), (1), (1)
Host Mouse
(130), (49), (9), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This DIABLO antibody is un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(177), (78), (68), (45), (29), (17), (11), (10), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
Supplier Log in to see

Product Details anti-DIABLO Antibody

Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN153019) Images
Purification Ascites
Immunogen Recombinant mature human Smac (lacks amino acids 1-55 present in Smac precursor). [UniProt# Q9NR28]
Clone 78-1-118
Isotype IgG3, IgG1

Target Details DIABLO

Product Details anti-DIABLO Antibody Application Details Handling References for anti-DIABLO Antibody (ABIN153019) Images back to top
Antigen
Alternative Name SMAC/Diablo (DIABLO Antibody Abstract)
Background Gene Symbol: DIABLO
Molecular Weight Theoretical MW: 25 kDa
Gene ID 56616, 66593, 288753
UniProt Q9NR28
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-DIABLO Antibody Target Details DIABLO Handling References for anti-DIABLO Antibody (ABIN153019) Images back to top
Application Notes Western Blot 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunoprecipitation protocol specific for Smac Antibody Procedure Guide for NB 500-213 Monoclonal anti-Survivin (clone 58.22)Immunoprecipitation Procedure1) Lyse cells plated in a 60mm dish:a) 300 µL CHAPS buffer [50mM Tris-HCl, pH 7.5/50mM NaCl/1mM EDTA/1 % NP-40/0.1 % CHAPS/1mM NaVO4/1mM PMSF]b) rock for 20 minutes at 4C2) Harvest lysate and spin down the insoluble material at 14K rpm. 3) Collect soluble fraction.
4) Pre-clear lysate with 40 µL of 50:50 slurry of Protein A beads, rocking for 1 hour at 4C.
) Spin down beads at 2K rpm, at 4C.
) Collect pre-cleared lysate.
) Incubate lysate with 5-7ug of anti-Survivin overnight, rocking at 4C. 8) Add 50 µL of Protein A 50:50 slurry for 2 hours, rocking at 4C.
) Wash beads with 200 µL of CHAPS buffer, three times.
) Denature immune complex by adding 2x Sample
Restrictions For Research Use only

Handling

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details References for anti-DIABLO Antibody (ABIN153019) Images back to top
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-DIABLO Antibody (ABIN153019)

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling Images back to top
Product cited in:

Hwang, Dornbos, Steidemann, Dunivin, Rizzo, LaPres: "Mitochondrial-targeted aryl hydrocarbon receptor and the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome." in: Toxicology and applied pharmacology, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sirianni, Jiang, Zeng, Mao, Zhou, Sugarbaker, Zhang, Li, Friedlander, Wang: "N-acetyl-l-tryptophan, but not N-acetyl-d-tryptophan, rescues neuronal cell death in models of amyotrophic lateral sclerosis." in: Journal of neurochemistry, Vol. 134, Issue 5, pp. 956-68, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Zhang, Cook, Kim, Baranov, Jiang, Smith, Cormier, Bennett, Browser, Day, Carlisle, Ferrante, Wang, Friedlander: "Melatonin inhibits the caspase-1/cytochrome c/caspase-3 cell death pathway, inhibits MT1 receptor loss and delays disease progression in a mouse model of amyotrophic lateral sclerosis." in: Neurobiology of disease, Vol. 55, pp. 26-35, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Wang, Sirianni, Pei, Cormier, Smith, Jiang, Zhou, Wang, Zhao, Yano, Kim, Li, Kristal, Ferrante, Friedlander: "The melatonin MT1 receptor axis modulates mutant Huntingtin-mediated toxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 41, pp. 14496-507, 2011

Zhang, Wang, Wang, Narayanan, Stavrovskaya, Kristal, Friedlander: "Nortriptyline protects mitochondria and reduces cerebral ischemia/hypoxia injury." in: Stroke; a journal of cerebral circulation, Vol. 39, Issue 2, pp. 455-62, 2008

Wang, Zhu, Pei, Drozda, Stavrovskaya, Del Signore, Cormier, Shimony, Wang, Ferrante, Kristal, Friedlander: "Inhibitors of cytochrome c release with therapeutic potential for Huntington's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 38, pp. 9473-85, 2008 Further details: Western Blotting

Liu, Deng, Lehal, Kim, Zacksenhaus: "Identification of tumorsphere- and tumor-initiating cells in HER2/Neu-induced mammary tumors." in: Cancer research, Vol. 67, Issue 18, pp. 8671-81, 2007

Wang, Zhu, Drozda, Zhang, Stavrovskaya, Cattaneo, Ferrante, Kristal, Friedlander: "Minocycline inhibits caspase-independent and -dependent mitochondrial cell death pathways in models of Huntington's disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 18, pp. 10483-7, 2003 Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Zhang, Wang, Narayanan, Zhang, Huo, Reed, Friedlander: "Fundamental role of the Rip2/caspase-1 pathway in hypoxia and ischemia-induced neuronal cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 26, pp. 16012-7, 2003 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product Details anti-DIABLO Antibody Target Details DIABLO Application Details Handling References for anti-DIABLO Antibody (ABIN153019) back to top
Supplier Images
Western Blotting (WB) image for anti-DIABLO antibody (Second Mitochondria-Derived Activator of Caspase) (ABIN153019) Western Blot: Smac Antibody (78-1-118) [ABIN153019] - HeLa whole cell extract lysates.