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anti-Human FASL antibody for Flow Cytometry

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Reactivity
Human, Mouse (Murine)
274
137
65
3
3
1
1
1
1
1
Host
Hamster
146
127
60
29
12
6
Clonality
Monoclonal
Conjugate
This FASL antibody is conjugated to Biotin
31
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
196
162
87
63
57
50
30
29
17
12
11
11
11
6
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1105946
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN120317 EIA FACS IP Mouse IgG1 Log in to see 1OF2 2
0.30226976 ABIN120318 EIA FACS IP Mouse IgG1 Log in to see 1OF2 2
0.30226976 ABIN1105942 FACS Hamster IgG Log in to see FLIM58 14
0.30226976 ABIN1105944 FACS Mouse IgG1 Log in to see B-R17 1
0.30226976 ABIN1105947 FACS FITC Hamster IgG Log in to see GE 4-90 6
0.30226976 ABIN1105948 FACS FITC Hamster IgG Log in to see FLIM58 14
0.30226976 ABIN1105949 FACS PE Hamster IgG Log in to see 4H11 5
0.30226976 ABIN120316 EIA Func FACS IP Mouse IgG1 Log in to see 1OF2 2
0.30226976 ABIN1105950 FACS Biotin Mouse IgG1 Log in to see B-R17
0.30226976 ABIN1177002 FACS IP Neut WB Mouse IgG1 Log in to see NOK-1 9
0.30226976 ABIN1105943 Func FACS Mouse IgG1 Log in to see B-R17
0.30226976 ABIN2145284 FACS Mouse IgG1 kappa Log in to see 4ANOK-1 2
0.30226976 ABIN2145282 FACS Mouse IgG1 kappa Log in to see 4ANOK-1 2
0.30226976 ABIN2660965 FACS Biotin Mouse IgG1 kappa Log in to see NOK-1 11
0.30226976 ABIN2665566 FACS Mouse IgG1 kappa Log in to see NOK-1 10
0.30226976 ABIN2663099 FACS CyTOF PE Mouse IgG1 kappa Log in to see NOK-1 7
0.30226976 ABIN2664298 FACS Mouse IgG1 kappa Log in to see NOK-1 10
0.30226976 ABIN262768 FACS ICC IF IP WB Mouse IgG1 kappa Log in to see NOK-1
0.30226976 ABIN2169456 FACS PE Mouse IgG1 Log in to see
0.30226976 ABIN187289 FACS IF WB Rat IgG2a Log in to see H11

Top referenced anti-FASL antibody for Flow Cytometry

Similar anti-FASL Antibodies

Application / Reactivity Human Mouse (Murine)
Western Blotting (WB) 132 Antibodies 45 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Neutralization (Neut) 3 Antibodies 8 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies 1 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 66 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies 17 Antibodies
Immunohistochemistry (IHC) 27 Antibodies 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 16 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Human, Mouse (Murine)
(274), (137), (65), (3), (3), (1), (1), (1), (1), (1)
Host Hamster
(146), (127), (60), (29), (12), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is conjugated to Biotin
(31), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(196), (162), (87), (63), (57), (50), (30), (29), (17), (12), (11), (11), (11), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 14 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN1105946) Images
Specificity This antibody reacts with mouse FasL of B6, C3H and MRL mice as well as Balb/c and DBA/1 mice, but does not detect gld-type mouse FasL mutant by flow cytometric analysis. We have tested the reactivity on flow cytometry using mouse cell line W40LFL, which is overexpressor of mouse FasL extracellular region (See ref. 11) for more information about this cell line).
Cross-Reactivity (Details) Species reactivity (tested):Mouse. Weakly with human.
Purification Protein A agarose
Immunogen W40LFL cells, which is overexpressed mouse FasL extracellular region
Clone FLIM58
Isotype IgG

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling References for anti-FASL Antibody (ABIN1105946) Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Background Fas ligand (FasL/CD178/CD95L) is a type II-membrane protein, whose N terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas, also termed Apo-1 or CD95, is a cell-surface type I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family. It is known that engagement of Fas by FasL results in apoptotic cell death. Binding of a trimeric FasL to Fas induces trimerization of Fas, and FADD/MORT1 binds to the trimerized Fas cytoplasmic region through the interaction of the respective death domains. Caspase-8 is then recruited to FADD/MORT1 through binding of each death effector domains (DED), which in turn may induce self-activation of the protease domain and eventually leads cell to apoptosis. FasL is predominantly expressed in activated T lymphocytes and Natural Killer (NK) cells, although it is expressed in the tissues of the immune-privilege sites such as the testis and eye. Furthermore, a variety of cancers such as melanomas were found to express FasL in addition to Fas. On the other hand, Fas is expressed in various tissues with abundant expression in the thymus, liver, heart, and kidney. It is reported that Fas/FasL system is involved in the deletion of activated or autoreactive T and B cells. Mature T cells of normal mice are known to die, after they accomplish their tasks. On the contrary, mature T cells from lpr and gld mice, which are deficient of Fas and FasL respectively, do not die after activation, and activated cells accumulate in the lymph nodes and spleens of these mice. Moreover T cell hybridomas activated in the presence of Fas-neutralizing molecule, do not die. In addition to T cells, the Fas-deficient mice accumulate B cells and have elevated levels of immunoglobulins (Ig) of various classes that include anti-ssDNA and anti-dsDNA antibodies. FasL can be used as an immune-suppressive agent. Rejection of transplants is mediated by activated T cells that may express functional Fas. If a transplanted tissue is engineered to express FasL or is co-transplanted with FasL expressing cells, the transplant may be tolerated. Also Fas/ FasL system has been shown to play a role in human diseases such as fulminant hepatitis, AIDS, cancers and other diseases involving CTL-induced tissue destruction.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Gene ID 356
NCBI Accession NP_000630
UniProt P48023
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling References for anti-FASL Antibody (ABIN1105946) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details References for anti-FASL Antibody (ABIN1105946) Images back to top
Concentration 20 µg/mL
Buffer PBS, 0.09 % NaN3, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2 - 8 °C.

References for anti-FASL Antibody (ABIN1105946)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Background publications

Imamura, Konaka, Matsumoto, Hasegawa, Fukui, Mukaida, Kinoshita, Suda: "Fas ligand induces cell-autonomous NF-kappaB activation and interleukin-8 production by a mechanism distinct from that of tumor necrosis factor-alpha." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 46415-23, 2004

Plath, Wilkinson, Stirling, Rapoport: "Interactions between Sec complex and prepro-alpha-factor during posttranslational protein transport into the endoplasmic reticulum." in: Molecular biology of the cell, Vol. 15, Issue 1, pp. 1-10, 2003

Margalit, Fishman, Berko, Engberg, Gross: "Chimeric beta2 microglobulin/CD3zeta polypeptides expressed in T cells convert MHC class I peptide ligands into T cell activation receptors: a potential tool for specific targeting of pathogenic CD8(+) T cells." in: International immunology, Vol. 15, Issue 11, pp. 1379-87, 2003

Fukui, Imamura, Umemura, Kawabe, Suda: "Pathogen-associated molecular patterns sensitize macrophages to Fas ligand-induced apoptosis and IL-1 beta release." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 4, pp. 1868-74, 2003

Nakamoto, Kaneko, Fan, Momoi, Tsutsui, Nakanishi, Kobayashi, Suda: "Prevention of hepatocellular carcinoma development associated with chronic hepatitis by anti-fas ligand antibody therapy." in: The Journal of experimental medicine, Vol. 196, Issue 8, pp. 1105-11, 2002

Saegusa, Ishimaru, Yanagi, Mishima, Arakaki, Suda, Saito, Hayashi: "Prevention and induction of autoimmune exocrinopathy is dependent on pathogenic autoantigen cleavage in murine Sjögren's syndrome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 2, pp. 1050-7, 2002

Roger, Chalifour, Lemieux, Duplay: "Cutting edge: Ly49A inhibits TCR/CD3-induced apoptosis and IL-2 secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 6-10, 2001

Asao, Okuyama, Kumaki, Ishii, Tsuchiya, Foster, Sugamura: "Cutting edge: the common gamma-chain is an indispensable subunit of the IL-21 receptor complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 1, pp. 1-5, 2001

Saito, Haruta, Shimuta, Inoue, Sakurai, Yamada, Ishimaru, Higashiyama, Sumida, Ishida, Suda, Noda, Hayashi, Tsubota: "Fas ligand-mediated exocrinopathy resembling Sjögren's syndrome in mice transgenic for IL-10." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 5, pp. 2488-94, 1999

Miwa, Hashimoto, Yatomi, Nakamura, Nagata, Suda: "Therapeutic effect of an anti-Fas ligand mAb on lethal graft-versus-host disease." in: International immunology, Vol. 11, Issue 6, pp. 925-31, 1999

Waring, Müllbacher: "Cell death induced by the Fas/Fas ligand pathway and its role in pathology." in: Immunology and cell biology, Vol. 77, Issue 4, pp. 312-7, 1999

Zeytun, Hassuneh, Nagarkatti, Nagarkatti: "Fas-Fas ligand-based interactions between tumor cells and tumor-specific cytotoxic T lymphocytes: a lethal two-way street." in: Blood, Vol. 90, Issue 5, pp. 1952-9, 1997

Hueber, Zörnig, Lyon, Suda, Nagata, Evan: "Requirement for the CD95 receptor-ligand pathway in c-Myc-induced apoptosis." in: Science (New York, N.Y.), Vol. 278, Issue 5341, pp. 1305-9, 1997

Kayagaki, Yamaguchi, Nagao, Matsuo, Maeda, Okumura, Yagita: "Polymorphism of murine Fas ligand that affects the biological activity." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3914-9, 1997

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling References for anti-FASL Antibody (ABIN1105946) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6))  (Biotin) (ABIN1105946) anti-Fas Ligand (TNF Superfamily, Member 6) (FASL) antibody (Biotin)