anti-Human FASL antibody for Flow Cytometry

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • fasl
  • FASL
  • FASLG
  • FAS
  • TNFRSF6
  • zgc:162027
  • TNFSF6
  • ALPS1B
  • APT1LG1
  • APTL
  • CD178
  • CD95-L
  • CD95L
  • Fas-L
  • Faslg
  • Tnfsf6
  • gld
  • Apt1Lg1
  • Fasl
  • Fas ligand (TNF superfamily, member 6)
  • Fas (TNF receptor superfamily, member 6)
  • fas ligand
  • faslg
  • FAS
  • FASLG
  • fas
  • LOC100344073
  • Fasl
  • Faslg
Reactivity
Human
366
172
89
22
7
5
5
3
3
2
1
1
Host
Mouse
203
174
49
32
29
8
1
Clonality
Monoclonal
Conjugate
This FASL antibody is un-conjugated
41
29
24
9
9
7
6
6
6
5
5
5
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP)
290
215
97
81
73
63
38
33
28
28
13
12
10
9
5
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120318
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.376486 ABIN120317 EIA FACS IP Mouse IgG1 Log in to see 1OF2 2
14.376486 ABIN1105946 FACS Biotin Hamster IgG Log in to see FLIM58 14
14.376486 ABIN1105948 FACS FITC Hamster IgG Log in to see FLIM58 14
14.376486 ABIN1105947 FACS FITC Hamster IgG Log in to see GE 4-90 6
14.376486 ABIN1105944 FACS Mouse IgG1 Log in to see B-R17 1
14.376486 ABIN1105942 FACS Hamster IgG Log in to see FLIM58 14
14.376486 ABIN1105949 FACS PE Hamster IgG Log in to see 4H11 5
14.376486 ABIN120316 EIA Func FACS IP Mouse IgG1 Log in to see 1OF2 2
14.376486 ABIN1105950 FACS Biotin Mouse IgG1 Log in to see B-R17
14.376486 ABIN4271438 CyTOF ELISA FACS WB Mouse IgG1 Log in to see 4F8D6 2
14.376486 ABIN1177002 FACS IP Neut WB Mouse IgG1 Log in to see NOK-1 9
14.376486 ABIN2660965 FACS Biotin Mouse IgG1 kappa Log in to see NOK-1 11
14.376486 ABIN1105943 Func FACS Mouse IgG1 Log in to see B-R17
14.376486 ABIN262768 FACS ICC IF IP WB Mouse IgG1 kappa Log in to see NOK-1
14.376486 ABIN254237 FACS IP ELISA Mouse IgG1 Log in to see 14C2
14.376486 ABIN254238 FACS IP ELISA Mouse IgG1 Log in to see 1OF2
14.376486 ABIN2663099 FACS CyTOF PE Mouse IgG1 kappa Log in to see NOK-1 7
14.376486 ABIN2665566 FACS Mouse IgG1 kappa Log in to see NOK-1 10
14.376486 ABIN2478842 FACS IP ELISA Mouse IgG1 Log in to see 10F2 3
14.376486 ABIN2478844 FACS IP ELISA Mouse IgG1 Log in to see 14C2 4

Top referenced anti-FASL antibody for Flow Cytometry

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Application / Reactivity Human
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General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Reactivity Human
(366), (172), (89), (22), (7), (5), (5), (3), (3), (2), (1), (1)
Host Mouse
(203), (174), (49), (32), (29), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FASL antibody is un-conjugated
(41), (29), (24), (9), (9), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP)
(290), (215), (97), (81), (73), (63), (38), (33), (28), (28), (13), (12), (10), (9), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling ProductDetails: References for anti-FASL antibody (ABIN120318) Images
Specificity This antibody recognises the CD178 cell surface antigen. This antibody may be used in combination with clone 14C2 (MCA2409) in ELISA assays. Clone 10F2 is reported to block the activity of Fas ligand. We recommend the use of SM2268LE for such studies.
Purification Affinity Chromatography on Protein G
Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the P3U1 myeloma cell line.
Clone 1OF2
Isotype IgG1

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling ProductDetails: References for anti-FASL antibody (ABIN120318) Images back to top
Antigen
Alternative Name CD178 / Fas Ligand (FASL Antibody Abstract)
Background CD178 is a 40 kDa glycoprotein also known as Fas ligand (CD95L). CD178 is a member of the TNF family, which is expressed by activated T lymphocytes and NK cells. The protein may exist as either a membrane bound or a cleaved soluble form. CD178 plays an important role in T cell development and cytotoxicity. Binding of CD178 to Fas (CD95) results in the induction of apoptosis.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6
Gene ID 356
NCBI Accession NP_000630
UniProt P48023
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling ProductDetails: References for anti-FASL antibody (ABIN120318) Images back to top
Application Notes Flow Cytometry: 1/25 - 1/50, Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details ProductDetails: References for anti-FASL antibody (ABIN120318) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-FASL antibody (ABIN120318)

Product Details anti-FASL Antibody Target Details FASL Application Details Handling Images back to top
Background publications

Legembre, Moreau, Daburon, Moreau, Taupin: "Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas." in: Cell death and differentiation, Vol. 9, Issue 3, pp. 329-39, 2002

Ferry-Dumazet, Garnier, Mamani-Matsuda, Vercauteren, Belloc, Billiard, Dupouy, Thiolat, Kolb, Marit, Reiffers, Mossalayi: "Resveratrol inhibits the growth and induces the apoptosis of both normal and leukemic hematopoietic cells." in: Carcinogenesis, Vol. 23, Issue 8, pp. 1327-33, 2002

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling ProductDetails: References for anti-FASL antibody (ABIN120318) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (ABIN120318) Staining of CD178 transfected CHO cells with MOUSE ANTI HUMAN CD178 (ABIN120318)