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anti-Human FASL antibody for Immunocytochemistry

Recommended FASL Antibody (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Epitope
AA 192-218, C-Term
29
24
14
12
11
9
8
6
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
274
138
65
3
3
1
1
1
1
1
Host
Rabbit
145
127
61
29
12
6
Clonality
Polyclonal
Conjugate
This FASL antibody is un-conjugated
32
26
18
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
197
161
87
63
57
49
29
29
17
12
11
11
11
6
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN3044333
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010086453 ABIN262768 FACS ICC IF IP WB Mouse IgG1 kappa Log in to see NOK-1
0.0010086453 ABIN609580 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010086453 ABIN1492926 ICC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010086453 ABIN205444 ICC FACS IF IP Mouse IgG1 Log in to see NOK-1
0.0010086453 ABIN2749018 Func ICC FACS IP WB Mouse IgG1 Log in to see NOK-1 5
0.0010086453 ABIN2749049 Func ICC FACS IP WB Mouse IgG1 Log in to see NOK-1 5
0.0010086453 ABIN1858802 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 103-281 Log in to see Polyclonal
0.0010086453 ABIN1172027 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 103-281 Log in to see Polyclonal
0.0010086453 ABIN199147 Func ICC FACS Neut Mouse IgG1 Extracellular Domain Log in to see
0.0010086453 ABIN1981904 Func ICC FACS IP WB PE Mouse IgG1 Log in to see NOK-1 5
0.0010086453 ABIN3199540 FACS Func ICC IP WB PE Mouse IgG1 Log in to see NOK-1
0.0010086453 ABIN3199538 FACS Func ICC IP WB Mouse IgG1 Log in to see NOK-1
0.0010086453 ABIN2140235 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010086453 ABIN3199539 FACS Func ICC IP WB Mouse IgG1 Log in to see NOK-1
0.0010086453 ABIN3030909 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
0.0010086453 ABIN3036805 Func ICC FACS IF IP WB Mouse IgG1 Log in to see
0.0010086453 ABIN3036807 Func ICC FACS IF IP WB PE Mouse IgG1 Log in to see
0.0010086453 ABIN3066921 ICC FACS IF Mouse IgG1 kappa Log in to see
0.0010086453 ABIN3036806 Func ICC FACS IF IP WB Mouse IgG1 Log in to see
0.0010086453 ABIN1689938 ICC IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-FASL antibody for Immunocytochemistry

Similar anti-FASL Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 2 Antibodies
Cell-ELISA (cELISA) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
ELISA 82 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot 1 Antibodies
Enzyme Immunoassay (EIA) 11 Antibodies
Flow Cytometry (FACS) 120 Antibodies
Functional Studies (Func) 23 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 21 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 66 Antibodies

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies
Epitope AA 192-218, C-Term
(29), (24), (14), (12), (11), (9), (8), (6), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(274), (138), (65), (3), (3), (1), (1), (1), (1), (1)
Host Rabbit
(145), (127), (61), (29), (12), (6)
Clonality
Polyclonal
Conjugate This FASL antibody is un-conjugated
(32), (26), (18), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(197), (161), (87), (63), (57), (49), (29), (29), (17), (12), (11), (11), (11), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-FASL Antibody

Target Details FASL Application Details Handling Images
Sequence YSKVYFRGQS CNNLPLSHKV YMRNSKY
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: Fas ligand(TNF superfamily, member 6)
Protein Name: Tumor necrosis factor ligand superfamily member 6
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(192-218aa YSKVYFRGQSCNNLPLSHKVYMRNSKY), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
Isotype IgG

Target Details FASL

Product Details anti-FASL Antibody Application Details Handling Images back to top
Antigen
Alternative Name FASLG (FASL Antibody Abstract)
Background FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
UniProt P48023
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details anti-FASL Antibody Target Details FASL Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-FASL Antibody Target Details FASL Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-FASL Antibody Target Details FASL Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (AA 192-218) (ABIN3044333) Anti-Fas Ligand antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue
Western Blotting (WB) image for anti-FASL antibody (Fas Ligand (TNF Superfamily, Member 6)) (AA 192-218) (ABIN3044333) Anti-Fas Ligand antibody, Western blotting Lane 1: MCF-7 Cell Lysate Lane 2: HELA Cel...