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|Application / Reactivity||Human|
|Blocking Antibody (Inhibition)||2 Antibodies|
|Cell-ELISA (cELISA)||1 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Dot Blot (DB)||1 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||11 Antibodies|
|Flow Cytometry (FACS)||120 Antibodies|
|Functional Studies (Func)||23 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies|
|Immunofluorescence (IF)||16 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||40 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||42 Antibodies|
|Immunoprecipitation (IP)||66 Antibodies|
|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) Antibodies|
|Epitope||AA 192-218, C-Term Alternatives|
|Conjugate||This FASL antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-FASL AntibodyTarget Details FASL Application Details Handling Images
|Sequence||YSKVYFRGQS CNNLPLSHKV YMRNSKY|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: Fas ligand(TNF superfamily, member 6)
Protein Name: Tumor necrosis factor ligand superfamily member 6
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(192-218aa YSKVYFRGQSCNNLPLSHKVYMRNSKY), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.|
Target Details FASLProduct Details anti-FASL Antibody Application Details Handling Images back to top
|Alternative Name||FASLG (FASL Antibody Abstract)|
|Background||FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.|
|Pathways||Apoptosis, EGFR Signaling Pathway|
Application DetailsProduct Details anti-FASL Antibody Target Details FASL Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FASL Antibody Target Details FASL Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
ImagesProduct Details anti-FASL Antibody Target Details FASL Application Details Handling back to top