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|Application / Reactivity||Rat (Rattus)|
|ELISA||18 ELISA Kits|
|Antigen||Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kits|
|Reactivity||Rat (Rattus) Alternatives|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||31.2-2000 pg/mL|
|Minimum Detection Limit||31.2 pg/mL|
|4 references available|
|Supplier||Log in to see|
Product Details FASL ELISA KitTarget details Application Details Handling References for FASL Kit (ABIN1672899) Images
|Purpose||For quantitative detection of rat FAS-L in cell culture supernates, serum and plasma(heparin, EDTA, citrate).|
|Sample Type||Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)|
|Specificity||Natural and recombinant rat FAS-L|
|Cross-Reactivity (Details)||There is no detectable cross-reactivity with other relevant proteins.|
|Material not included||Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl|
Expression system for standard: NSO
Immunogen sequence: Q127-L278
Target detailsProduct Details FASL ELISA Kit Application Details Handling References for FASL Kit (ABIN1672899) Images back to top
|Background||FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.|
|Pathways||Apoptosis, EGFR Signaling Pathway|
Application DetailsProduct Details FASL ELISA Kit Target details Handling References for FASL Kit (ABIN1672899) Images back to top
|Application Notes||Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.|
|Plate||Pre-coated,Strips (12 x 8)|
|Protocol||rat FAS-L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FAS-L has been precoated onto 96-well plates. Standards(NSO, Q127-L278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS-L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat FAS-L amount of sample captured in plate.|
|Assay Procedure||Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat FAS-L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat FAS-L standard solution and each sample be measured in duplicate.|
|Restrictions||For Research Use only|
HandlingProduct Details FASL ELISA Kit Target details Application Details References for FASL Kit (ABIN1672899) Images back to top
|Handling Advice||Avoid multiple freeze-thaw cycles.|
|Storage||-20 °C,4 °C|
|Storage Comment||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles|
|Expiry Date||12 months|
References for FASL Kit (ABIN1672899)Product Details FASL ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Jiang, Li, Zhou et al.: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, 2014 (PubMed).
Villa-Morales, Santos, Pérez-Gómez et al.: "A role for the Fas/FasL system in modulating genetic susceptibility to T-cell lymphoblastic lymphomas." in: Cancer research, Vol. 67, Issue 11, pp. 5107-16, 2007 (PubMed).
Zhang, Miao, Sun et al.: "Functional polymorphisms in cell death pathway genes FAS and FASL contribute to risk of lung cancer." in: Journal of medical genetics, Vol. 42, Issue 6, pp. 479-84, 2005 (PubMed).
Takahashi, Tanaka, Inazawa et al.: "Human Fas ligand: gene structure, chromosomal location and species specificity." in: International immunology, Vol. 6, Issue 10, pp. 1567-74, 1995 (PubMed).
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