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Rat (Rattus) FASL ELISA Kit

Recommended FASL Product (supplied by: Log in to see )

Antigen
Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kits
  • ALPS1B
  • APT1LG1
  • Apt1Lg1
  • APTL
  • CD95-L
  • CD95L
  • CD178
  • FAS
  • Fas-L
  • FASL
  • fasl
  • Fasl
  • Faslg
  • FASLG
  • gld
  • TNFRSF6
  • Tnfsf6
  • TNFSF6
  • zgc:162027
Epitope
AA 127-278
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
57
22
17
6
3
3
2
2
2
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
31.2-2000 pg/mL
Minimum Detection Limit
31.2 pg/mL
Application
ELISA
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Catalog No. ABIN1672899
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32670403 ABIN416309 Serum, Plasma, Tissue Homogenate, Biological Fluids 7.81-500 pg/mL 7.81 pg/mL Log in to see
0.32670403 ABIN1114849 Sandwich ELISA Serum, Plasma, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32670403 ABIN1569722 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.32670403 ABIN1888508 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.32670403 ABIN2708627 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.001210097 ABIN366478 Sandwich ELISA Serum, Plasma, Tissue Homogenate 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001210097 ABIN3032865 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 0.082-60 ng/mL 0.082 ng/mL Log in to see
0.001210097 ABIN625448 Sandwich ELISA Plasma, Serum, Cell Culture Supernatant, Cell and Tissue Culture Lysate, Body Fluids 90-60000 pg/mL 90 pg/mL Log in to see
0.001210097 ABIN627004 Sandwich ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids Log in to see
0.001210097 ABIN510674 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.001210097 ABIN2042459 Sandwich ELISA 31.2-2000 pg/mL 31.2 pg/mL Log in to see
0.001210097 ABIN579300 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.001210097 ABIN2684236 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum Log in to see
0.001210097 ABIN2533228 Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 1.5-100 pg/mL 1.5 pg/mL Log in to see
0.001210097 ABIN2082478 Sandwich ELISA Log in to see
0.001210097 ABIN2947377 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.001210097 ABIN2947373 7.81-500 pg/mL 7.81 pg/mL Log in to see

Top referenced FASL ELISA Kit for ELISA

Similar FASL ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 18 ELISA Kits

General

Antigen Fas Ligand (TNF Superfamily, Member 6) (FASL) ELISA Kits
Epitope AA 127-278
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(57), (22), (17), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 31.2-2000 pg/mL
Minimum Detection Limit 31.2 pg/mL
Application ELISA
Pubmed 4 references available
Supplier Log in to see

Product Details FASL ELISA Kit

Target details Application Details Handling References for FASL Kit (ABIN1672899) Images
Purpose For quantitative detection of rat FAS-L in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
Detection Method Colorimetric
Specificity Natural and recombinant rat FAS-L
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- rat FAS-L antibod - 1
  • Lyophilized recombinant rat FAS-L standar - 10ng/tubex2
  • Biotinylated anti- rat FAS-L antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: Q127-L278

Target details

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Antigen
Alternative Name FASLG (FASL ELISA Kit Abstract)
Background FAS Ligand(FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
Gene ID 25385
UniProt P36940
Pathways Apoptosis, EGFR Signaling Pathway

Application Details

Product Details FASL ELISA Kit Target details Handling References for FASL Kit (ABIN1672899) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol rat FAS-L ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FAS-L has been precoated onto 96-well plates. Standards(NSO, Q127-L278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FAS-L is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat FAS-L amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat FAS-L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat FAS-L standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 118, Standard deviation: 4.13, CV(%): 3.5
  • Sample 2: n=16, Mean(pg/ml): 854, Standard deviation: 40.99, CV(%): 4.8
  • Sample 3: n=16, Mean(pg/ml): 1319, Standard deviation: 71.23, CV(%): 5.4,
  • Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 5.2, CV(%): 5.1
  • Sample 2: n=24, Mean(pg/ml): 847, Standard deviation: 52.51, CV(%): 6.2
  • Sample 3: n=24, Mean(pg/ml): 1423, Standard deviation: 103.9, CV(%): 7.3
Restrictions For Research Use only

Handling

Product Details FASL ELISA Kit Target details Application Details References for FASL Kit (ABIN1672899) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for FASL Kit (ABIN1672899)

Product Details FASL ELISA Kit Target details Application Details Handling Images back to top
Product cited in:

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, 2014

Background publications

Villa-Morales, Santos, Pérez-Gómez, Quintanilla, Fernández-Piqueras: "A role for the Fas/FasL system in modulating genetic susceptibility to T-cell lymphoblastic lymphomas." in: Cancer research, Vol. 67, Issue 11, pp. 5107-16, 2007

Zhang, Miao, Sun, Tan, Qu, Xiong, Zhou, Lin: "Functional polymorphisms in cell death pathway genes FAS and FASL contribute to risk of lung cancer." in: Journal of medical genetics, Vol. 42, Issue 6, pp. 479-84, 2005

Takahashi, Tanaka, Inazawa, Abe, Suda, Nagata: "Human Fas ligand: gene structure, chromosomal location and species specificity." in: International immunology, Vol. 6, Issue 10, pp. 1567-74, 1995

Images

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Supplier Images
ELISA image for FASL ELISA Kit (Fas Ligand (TNF Superfamily, Member 6)) (ABIN1672899) Rat FAS-L PicoKine ELISA Kit standard curve