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|Antigen||Keratin 18 (KRT18) Antibodies|
|Conjugate||This KRT18 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-KRT18 AntibodyTarget Details KRT18 Application Details Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images
|Predicted Reactivity||Cow (Bovine),Frog,Mouse (Murine),Rat (Rattus)|
|Characteristics||Cellular Localization: Cytoplasm|
|Purification||Purified by Protein A/G|
|Immunogen||Synthetic peptide corresponding to C-terminus of human Keratin 18.|
Target Details KRT18Product Details anti-KRT18 Antibody Application Details Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images back to top
|Alternative Name||Keratin 18 (KRT18 Antibody Abstract)|
|Background||Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with cytokeratin 8, a basic keratin. Hepatocellular carcinomas express only cytokeratins 8 and 18.|
|Molecular Weight||52 kDa|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-KRT18 Antibody Target Details KRT18 Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images back to top
|Application Notes||IHC Dilution: 1:200|
IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
|Restrictions||For Research Use only|
HandlingProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images back to top
|Buffer||rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.|
|Precaution of Use||
1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
|Handling Advice||Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.|
ProductDetails: References for anti-KRT18 antibody (ABIN1687808)Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
|Product cited in:||
Xiang, Hurchla, Fontana, Su, Amend, Esser, Douglas, Mudalagiriyappa, Luker, Pluard, Ademuyiwa, Romagnoli, Tuffin, Chevalier, Luker, Bauer, Zimmermann, Aft, Dembowsky, Weilbaecher: "CXCR4 Protein Epitope Mimetic Antagonist POL5551 Disrupts Metastasis and Enhances Chemotherapy Effect in Triple-Negative Breast Cancer." in: Molecular cancer therapeutics, Vol. 14, Issue 11, pp. 2473-85, 2015
Krasteva-Christ, Soultanova, Schütz, Papadakis, Weiss, Deckmann, Chubanov, Gudermann, Voigt, Meyerhof, Boehm, Weihe, Kummer: "Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts." in: International immunopharmacology, Vol. 29, Issue 1, pp. 158-65, 2015
ImagesProduct Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) back to top