anti-Human KRT18 antibody for Flow Cytometry

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • CYK18
  • K18
  • CK18
  • Krt1-18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krtt1c6
  • zgc:66015
  • keratin 18
  • similar to Keratin, type I cytoskeletal 18 (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
  • Keratin, type I cytoskeletal 18
  • cytokeratin 18
  • K18, simple type I keratin
  • zgc:77517
  • krt18
  • KRT18
  • k1c18
  • LOC100008885
  • LOC100136778
  • Krt18
  • zgc:77517
Epitope
C-Term
51
36
27
23
20
16
15
11
11
10
9
8
7
6
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
947
200
171
71
65
41
36
34
27
27
23
20
18
15
12
7
7
5
5
4
3
3
2
2
1
1
1
Host
Rabbit
699
274
12
11
4
3
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
52
48
22
18
17
17
14
14
14
14
14
14
14
14
14
11
11
8
4
3
3
3
3
3
3
3
3
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
655
466
388
349
331
300
225
180
125
40
26
18
18
14
11
9
3
2
2
1
1
1
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Catalog No. ABIN1687808
$ 672.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.320047 ABIN676973 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 70-110 Log in to see Polyclonal 2
14.320047 ABIN268940 CyTOF ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 Log in to see C-04 1
14.320047 ABIN269268 FACS IHC IHC (p) WB Biotin Mouse IgG1 Log in to see C-04
14.320047 ABIN3031537 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 401-430 Log in to see Polyclonal
1 ABIN272330 FACS IF IHC (p) FITC Mouse IgG1 Log in to see DC-10 3
1 ABIN4302637 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 Log in to see DC10 2
1 ABIN1106919 IHC (fro) FACS IF IHC (p) WB Mouse IgG1 Log in to see RCK106 10
1 ABIN1106920 IHC (fro) FACS IF IHC (p) WB Mouse IgG1 Log in to see DE-K18 4
1 ABIN1106918 IHC (fro) FACS IF WB Mouse IgG1 Log in to see RGE53 9
1 ABIN391561 FACS IF IHC (p) WB Rabbit Ig AA 401-430, C-Term Log in to see Polyclonal
1 ABIN356293 FACS IHC (fro) IF IHC (p) FITC Mouse IgG1 Log in to see CK209 2
1 ABIN94282 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see DC-10 4
1 ABIN94283 ELISA FACS ICC IHC (p) IP WB Biotin Mouse IgG1 Log in to see DC-10 3
1 ABIN302009 ELISA FACS ICC IHC (p) IP WB FITC Mouse IgG1 Log in to see DC-10 3
1 ABIN356294 FACS IHC (fro) IF IHC (p) Mouse IgG1 Log in to see CK209 2
1 ABIN959458 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see DE-K18
1 ABIN2656840 FACS Alexa Fluor 594 Mouse IgG1 kappa Log in to see DA-7 5
1 ABIN2657197 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see DA-7 5
1 ABIN4302603 CyTOF FACS ICC IF IHC IHC (fro) WB Mouse IgG1 Log in to see RGE53
1 ABIN462333 FACS IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see Ks 18-04

General

Antigen Keratin 18 (KRT18) Antibodies
Epitope C-Term
(51), (36), (27), (23), (20), (16), (15), (11), (11), (10), (9), (8), (7), (6), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(947), (200), (171), (71), (65), (41), (36), (34), (27), (27), (23), (20), (18), (15), (12), (7), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(699), (274), (12), (11), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(52), (48), (22), (18), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (14), (11), (11), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(655), (466), (388), (349), (331), (300), (225), (180), (125), (40), (26), (18), (18), (14), (11), (9), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images
Predicted Reactivity Cow (Bovine),Frog,Mouse (Murine),Rat (Rattus)
Characteristics Cellular Localization: Cytoplasm
Purification Purified by Protein A/G
Immunogen Synthetic peptide corresponding to C-terminus of human Keratin 18.
Clone SP69
Isotype IgG

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images back to top
Antigen
Alternative Name Keratin 18 (KRT18 Antibody Abstract)
Background Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with cytokeratin 8, a basic keratin. Hepatocellular carcinomas express only cytokeratins 8 and 18.
Molecular Weight 52 kDa
Gene ID 3875
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images back to top
Application Notes IHC Dilution: 1:200
Comment

Positive Control:
IHC (p): Prostate, breast adenocarcinoma
WB: A431 cell lysate
FC: HeLa Cell Line

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details ProductDetails: References for anti-KRT18 antibody (ABIN1687808) Images back to top
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

ProductDetails: References for anti-KRT18 antibody (ABIN1687808)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Xiang, Hurchla, Fontana, Su, Amend, Esser, Douglas, Mudalagiriyappa, Luker, Pluard, Ademuyiwa, Romagnoli, Tuffin, Chevalier, Luker, Bauer, Zimmermann, Aft, Dembowsky, Weilbaecher: "CXCR4 Protein Epitope Mimetic Antagonist POL5551 Disrupts Metastasis and Enhances Chemotherapy Effect in Triple-Negative Breast Cancer." in: Molecular cancer therapeutics, Vol. 14, Issue 11, pp. 2473-85, 2015

Krasteva-Christ, Soultanova, Schütz, Papadakis, Weiss, Deckmann, Chubanov, Gudermann, Voigt, Meyerhof, Boehm, Weihe, Kummer: "Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts." in: International immunopharmacology, Vol. 29, Issue 1, pp. 158-65, 2015

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling ProductDetails: References for anti-KRT18 antibody (ABIN1687808) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) Flow cytometric analysis of rabbit anti-Keratin 18 (SP69) antibody in HeLa (green) co...
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 7)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 8)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 9)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 10)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 11)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 12)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 13)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 14)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 15)
Immunohistochemistry (IHC) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 16)
Western Blotting (WB) image for anti-Keratin 18 (KRT18) (C-Term) antibody (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 17)