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anti-Human KRT18 antibody for Flow Cytometry

Recommended KRT18 Antibody (supplied by: Log in to see )

Antigen
Keratin 18 (KRT18) Antibodies
  • krt18
  • MGC64569
  • MGC75922
  • KRT18
  • CYK18
  • K18
  • CK18
  • Krt1-18
  • DreK18
  • cb83
  • dapk1
  • sb:cb83
  • wu:fa13f02
  • wu:fb36b04
  • krtt1c6
  • zgc:66015
  • keratin 18
  • similar to Keratin, type I cytoskeletal 18 (Cytokeratin-18) (CK-18) (Keratin-18) (K18)
  • Keratin, type I cytoskeletal 18
  • cytokeratin 18
  • K18, simple type I keratin
  • zgc:77517
  • krt18
  • KRT18
  • k1c18
  • LOC100008885
  • LOC100136778
  • Krt18
  • zgc:77517
Epitope
C-Term
51
33
25
24
21
16
15
11
10
10
9
8
7
6
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
916
200
172
73
66
41
37
37
27
24
22
22
19
13
10
7
7
5
5
5
3
3
2
2
1
1
1
Host
Rabbit
671
271
12
11
6
2
Clonality
Monoclonal
Conjugate
This KRT18 antibody is un-conjugated
51
48
23
18
17
17
14
14
14
14
14
14
14
14
14
11
11
8
4
3
3
3
3
3
3
3
3
2
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
637
427
365
348
309
302
215
177
119
40
26
18
18
13
12
9
3
2
2
1
1
1
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Catalog No. ABIN1687808
$ 672.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.411841 ABIN676973 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 70-110 Log in to see Polyclonal 2
13.411841 ABIN3031537 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 401-430 Log in to see Polyclonal
13.411841 ABIN2489420 FACS IHC ELISA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
1 ABIN272330 FACS IF IHC (p) FITC Mouse IgG1 Log in to see DC-10 3
1 ABIN4302637 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP SimWes WB Mouse IgG1 Log in to see DC10 2
1 ABIN1106919 IHC (fro) FACS IF IHC (p) WB Mouse IgG1 Log in to see RCK106 10
1 ABIN1106920 IHC (fro) FACS IF IHC (p) WB Mouse IgG1 Log in to see DE-K18 4
1 ABIN111182 FACS IHC (fro) IF IHC (p) FITC Mouse IgG1 Log in to see Ks18-04 9
1 ABIN268940 CyTOF ELISA FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 Log in to see C-04 1
1 ABIN1106918 IHC (fro) FACS IF WB Mouse IgG1 Log in to see RGE53 9
1 ABIN356293 FACS IHC (fro) IF IHC (p) FITC Mouse IgG1 Log in to see CK209 2
1 ABIN94283 ELISA FACS ICC IHC (p) IP WB Biotin Mouse IgG1 Log in to see DC-10 3
1 ABIN94282 ICC FACS IHC (p) IP ELISA WB Mouse IgG1 Log in to see DC-10 4
1 ABIN356294 FACS IHC (fro) IF IHC (p) Mouse IgG1 Log in to see CK209 2
1 ABIN302009 ELISA FACS ICC IHC (p) IP WB FITC Mouse IgG1 Log in to see DC-10 3
1 ABIN452157 FACS IHC (p) WB Rabbit IgG C-Term Log in to see SP69
1 ABIN2657197 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see DA-7 5
1 ABIN2656840 FACS Alexa Fluor 594 Mouse IgG1 kappa Log in to see DA-7 5
1 ABIN391561 FACS IF IHC (p) WB Rabbit Ig AA 401-430, C-Term Log in to see Polyclonal
1 ABIN462410 Inhibition ELISA FACS ICC IHC (p) IP WB Mouse IgG1 Log in to see DC-10

Top referenced anti-KRT18 antibody for Flow Cytometry

Similar anti-KRT18 Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
Blocking Antibody (Inhibition) 3 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies
ELISA 277 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 354 Antibodies
Immunochromatography (IC) 18 Antibodies
Immunocytochemistry (ICC) 201 Antibodies
Immunofluorescence (IF) 307 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 20 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 327 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 176 Antibodies

General

Antigen Keratin 18 (KRT18) Antibodies
Epitope C-Term
(51), (33), (25), (24), (21), (16), (15), (11), (10), (10), (9), (8), (7), (6), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(916), (200), (172), (73), (66), (41), (37), (37), (27), (24), (22), (22), (19), (13), (10), (7), (7), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1)
Host Rabbit
(671), (271), (12), (11), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This KRT18 antibody is un-conjugated
(51), (48), (23), (18), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (14), (11), (11), (8), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(637), (427), (365), (348), (309), (302), (215), (177), (119), (40), (26), (18), (18), (13), (12), (9), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-KRT18 Antibody

Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN1687808) Images
Predicted Reactivity Cow (Bovine),Frog,Mouse (Murine),Rat (Rattus)
Characteristics Cellular Localization: Cytoplasm
Purification Purified by Protein A/G
Immunogen Synthetic peptide corresponding to C-terminus of human Keratin 18.
Clone SP69
Isotype IgG

Target Details KRT18

Product Details anti-KRT18 Antibody Application Details Handling References for anti-KRT18 Antibody (ABIN1687808) Images back to top
Antigen
Alternative Name Keratin 18 (KRT18 Antibody Abstract)
Background Cytokeratin 18 is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with cytokeratin 8, a basic keratin. Hepatocellular carcinomas express only cytokeratins 8 and 18.
Molecular Weight 52 kDa
Gene ID 3875
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-KRT18 Antibody Target Details KRT18 Handling References for anti-KRT18 Antibody (ABIN1687808) Images back to top
Application Notes IHC Dilution: 1:200
Comment

Positive Control:
IHC (p): Prostate, breast adenocarcinoma
WB: A431 cell lysate
FC: HeLa Cell Line

Protocol IHC Procedure: Specimen
Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody.
Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.
Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:200. The dilutions are estimates, actual results may differ because of variability in methods and protocols.
Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Primary Antibody Incubation: Incubate for 10 minutes at room temperature.
Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05 % Tween.
Visualization: Detect the antibody as instructed by the instructions provided with the visualization system. IHC
Restrictions For Research Use only

Handling

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details References for anti-KRT18 Antibody (ABIN1687808) Images back to top
Format Liquid
Buffer rabbit monoclonal antibody purified by protein A/G in PBS/1 % BSA buffer pH 7.6 with less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use 1. Avoid contact of reagents with eyes and mucous membranes. If reagents come into contact with sensitive areas, wash with copious amounts of water.
2. This product is harmful if swallowed.
3. Consult local or state authorities with regard to recommended method of disposal.
4. Avoid microbial contamination of reagents.
This product contains Sodium AzideTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product, therefore, positive and negative controls should be tested simultaneously with unknown specimens.
Storage 4 °C

References for anti-KRT18 Antibody (ABIN1687808)

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling Images back to top
Product cited in:

Xiang, Hurchla, Fontana, Su, Amend, Esser, Douglas, Mudalagiriyappa, Luker, Pluard, Ademuyiwa, Romagnoli, Tuffin, Chevalier, Luker, Bauer, Zimmermann, Aft, Dembowsky, Weilbaecher: "CXCR4 Protein Epitope Mimetic Antagonist POL5551 Disrupts Metastasis and Enhances Chemotherapy Effect in Triple-Negative Breast Cancer." in: Molecular cancer therapeutics, Vol. 14, Issue 11, pp. 2473-85, 2015

Krasteva-Christ, Soultanova, Schütz, Papadakis, Weiss, Deckmann, Chubanov, Gudermann, Voigt, Meyerhof, Boehm, Weihe, Kummer: "Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts." in: International immunopharmacology, Vol. 29, Issue 1, pp. 158-65, 2015

Images

Product Details anti-KRT18 Antibody Target Details KRT18 Application Details Handling References for anti-KRT18 Antibody (ABIN1687808) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) Flow cytometric analysis of rabbit anti-Keratin 18 (SP69) antibody in HeLa (green) co...
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 4)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 6)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 7)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 8)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 9)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 10)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 11)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 12)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 13)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 14)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 15)
Immunohistochemistry (IHC) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 16)
Western Blotting (WB) image for anti-KRT18 antibody (Keratin 18) (C-Term) (ABIN1687808) anti-Keratin 18 (KRT18) (C-Term) antibody (Image 17)