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anti-Rabbit Lamin B1 antibody for Enzyme Immunoassay

Recommended Lamin B1 Antibody (supplied by: Log in to see )

Antigen
Lamin B1 (LMNB1) Antibodies
  • ADLD
  • fc06g01
  • lamin-b
  • Lamin-L(I)
  • LMN
  • lmn
  • LMN2
  • lmn2
  • LMNB
  • lmnb
  • LMNB1
  • MGC52550
  • wu:fc06g01
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
125
52
47
10
7
6
5
4
4
3
2
1
1
1
Host
Mouse
81
34
19
Clonality
Monoclonal
Conjugate
This Lamin B1 antibody is un-conjugated
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
126
60
41
28
23
20
16
11
10
4
2
1
1
1
1
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Catalog No. ABIN360850
$ 390.50
Plus shipping costs $45.00

Top referenced anti-Lamin B1 antibody for Enzyme Immunoassay

Similar anti-Lamin B1 Antibodies

Application / Reactivity Mouse (Murine) Rat (Rattus) Sheep (Ovine) Rabbit Cow (Bovine) Dog (Canine) Human Zebrafish (Danio rerio)
Western Blotting (WB) 51 Antibodies 46 Antibodies 6 Antibodies 7 Antibodies 11 Antibodies 8 Antibodies 120 Antibodies 5 Antibodies
Immunoprecipitation (IP) 18 Antibodies 12 Antibodies 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 25 Antibodies 8 Antibodies 7 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 20 Antibodies 21 Antibodies 2 Antibodies

General

Antigen Lamin B1 (LMNB1) Antibodies
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine), Zebrafish (Danio rerio)
(125), (52), (47), (10), (7), (6), (5), (4), (4), (3), (2), (1), (1), (1)
Host Mouse
(81), (34), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Lamin B1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(126), (60), (41), (28), (23), (20), (16), (11), (10), (4), (2), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-Lamin B1 Antibody

Target Details Lamin B1 Application Details Handling References for anti-Lamin B1 Antibody (ABIN360850) Images
Specificity The antibody Clone 119D5-F1 reacts with an epitope located C-terminal of residue 231 in Lamin B1.
Purification Purified
Immunogen Purified Rat liver lamins.
Clone 119D5-F1
Isotype IgG1

Target Details Lamin B1

Product Details anti-Lamin B1 Antibody Application Details Handling References for anti-Lamin B1 Antibody (ABIN360850) Images back to top
Antigen
Alternative Name Lamin-B1 (LMNB1) (LMNB1 Antibody Abstract)
Background Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.Synonyms: LMN2, LMNB, LMNB-1, LMNB1, Lamin-B1, Nuclear Envelope Marker
Molecular Weight 67 kDa (Predicted)
Gene ID 4001
NCBI Accession NP_005564
UniProt P20700
Research Area Cytoskeleton
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Handling References for anti-Lamin B1 Antibody (ABIN360850) Images back to top
Application Notes Immunocytochemistry (1: 100 - 1: 200 with avidin-biotinylated horseradish peroxidasecomplex (ABC) as detection reagent). Immunoblotting (1: 100 - 1: 1000). Flow cytometry (1: 100 - 1: 200). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details References for anti-Lamin B1 Antibody (ABIN360850) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Lamin B1 Antibody (ABIN360850)

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details Handling Images back to top
Background publications

Broers, Bronnenberg, Kuijpers, Schutte, Hutchison, Ramaekers: "Partial cleavage of A-type lamins concurs with their total disintegration from the nuclear lamina during apoptosis." in: European journal of cell biology, Vol. 81, Issue 12, pp. 677-91, 2003

Neri, Raymond, Giordano, Capitani, Martelli: "Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells." in: Journal of cellular physiology, Vol. 178, Issue 3, pp. 284-95, 1999

Broers, Machiels, Kuijpers, Smedts, van den Kieboom, Raymond, Ramaekers: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997

Machiels, Ramaekers, Kuijpers, Groenewoud, Oosterhuis, Looijenga: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997

Pugh, Coates, Lane, Raymond, Quinlan: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997

Jansen, Machiels, Hopman, Broers, Bot, Arends, Ramaekers, Schouten: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997

Weaver, Carson, Walker, Chaly, Lach, Raymond, Brown, Sikorska: "Degradation of nuclear matrix and DNA cleavage in apoptotic thymocytes." in: Journal of cell science, Vol. 109 ( Pt 1), pp. 45-56, 1996

Machiels, Broers, Raymond, de Ley, Kuijpers, Caberg, Ramaekers: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996