anti-Rat (Rattus) Lamin B1 antibody for Immunohistochemistry (Frozen Sections)

Recommended Lamin B1 Antibody (supplied by: Log in to see )

Antigen
Lamin B1 (LMNB1) Antibodies
  • MGC52550
  • Lamin-L(I)
  • lamin-b
  • lmn
  • lmn2
  • lmnb
  • LMNB1
  • ADLD
  • LMN
  • LMN2
  • LMNB
  • fc06g01
  • wu:fc06g01
  • lamin B1
  • lmnb1
  • LMNB1
  • LOC100037680
  • Lmnb1
Epitope
AA 570-586, C-Term
20
11
10
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
152
71
61
16
14
14
9
6
5
4
4
3
3
2
1
1
1
1
Host
Rabbit
89
40
28
Clonality
Polyclonal
Conjugate
This Lamin B1 antibody is un-conjugated
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
146
64
48
31
30
24
15
14
9
5
4
2
1
1
1
Options
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Catalog No. ABIN3043687
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN360850 EIA FACS IHC (fro) IF WB Mouse IgG1 Log in to see 119D5-F1 8
1 ABIN4330073 ELISA FACS ICC IF IHC IHC (fro) WB Mouse IgG1 Log in to see 119D5-F1 1
1 ABIN1108013 IHC (fro) IF IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN126714 IHC (fro) IF WB Mouse IgG1 Log in to see X223 3
1 ABIN1960136 ICC IHC (fro) FACS ELISA WB Mouse IgG1 C-Term Log in to see 119D5-F1
1 ABIN3031586 ICC IHC (fro) IHC (p) WB Rabbit IgG AA 570-586 Log in to see Polyclonal
1 ABIN2963329 ICC IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 119D5-F1

General

Antigen Lamin B1 (LMNB1) Antibodies
Epitope AA 570-586, C-Term
(20), (11), (10), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(152), (71), (61), (16), (14), (14), (9), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Host Rabbit
(89), (40), (28)
Clonality
Polyclonal
Conjugate This Lamin B1 antibody is un-conjugated
(2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(146), (64), (48), (31), (30), (24), (15), (14), (9), (5), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-Lamin B1 Antibody

Target Details Lamin B1 Application Details Handling References for anti-Lamin B1 antibody (ABIN3043687) Images
Purpose Rabbit IgG polyclonal antibody for Lamin-B1(LMNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Sequence FHQQGTPRAS NRSCAIM
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Lamin-B1(LMNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: lamin B1
Protein Name: Lamin-B1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Lamin B1(570-586aa FHQQGTPRASNRSCAIM), different from the related rat sequence by one amino acid, and from the related mouse sequence by three amino acids.
Isotype IgG

Target Details Lamin B1

Product Details anti-Lamin B1 Antibody Application Details Handling References for anti-Lamin B1 antibody (ABIN3043687) Images back to top
Antigen
Alternative Name LMNB1 (LMNB1 Antibody Abstract)
Background Lamin-B1 is a protein that in humans is encoded by the LMNB1 gene. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are though to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Synonyms: ADLD antibody|lamin B1 antibody|Lamin-B1 antibody|LMN antibody|LMN2 antibody|LMNB antibody|Lmnb1 antibody|LMNB1_HUMAN antibody|MGC111419 antibody|OTTHUMP00000159218 antibody
UniProt P20700
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Handling References for anti-Lamin B1 antibody (ABIN3043687) Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staning of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details References for anti-Lamin B1 antibody (ABIN3043687) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-Lamin B1 antibody (ABIN3043687)

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details Handling Images back to top
Product cited in:

Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, 2014

Liu, Ma, Wu, Li, Zhang, Han, Wang, Reindl, Wu, Ma: "Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-?B signaling." in: BMC cancer, Vol. 14, pp. 686, 2014

Gao, Wu, Liu, Li, Song, Teng, Sheng, Liu, Yao, Chen, Jiang, Chen, Huang: "Interaction of NS2 with AIMP2 facilitates the switch from ubiquitination to SUMOylation of M1 in influenza A virus-infected cells." in: Journal of virology, Vol. 89, Issue 1, pp. 300-11, 2014

Images

Product Details anti-Lamin B1 Antibody Target Details Lamin B1 Application Details Handling References for anti-Lamin B1 antibody (ABIN3043687) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Lamin B1 (LMNB1) (AA 570-586), (C-Term) antibody (ABIN3043687) Anti-Lamin B1 antibody, IHC(F) IHC(F): Rat Intestine Tissue
Western Blotting (WB) image for anti-Lamin B1 (LMNB1) (AA 570-586), (C-Term) antibody (ABIN3043687) Anti-Lamin B1 antibody, Western blotting Lane 1: MCF-7 Cell Lysate Lane 2: HELA Cell ...
Immunohistochemistry (IHC) image for anti-Lamin B1 (LMNB1) (AA 570-586), (C-Term) antibody (ABIN3043687) Anti-Lamin B1 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-Lamin B1 (LMNB1) (AA 570-586), (C-Term) antibody (ABIN3043687) Anti-Lamin B1 antibody, ICC ICC: A549 Cell