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Rat (Rattus) Lamin B1 ELISA Kit

Recommended Lamin B1 Product (supplied by: Log in to see )

Antigen
Lamin B1 (LMNB1) ELISA Kits
  • MGC52550
  • Lamin-L(I)
  • lamin-b
  • lmn
  • lmn2
  • lmnb
  • LMNB1
  • ADLD
  • LMN
  • LMN2
  • LMNB
  • fc06g01
  • wu:fc06g01
  • lamin B1
  • lmnb1
  • LMNB1
  • LOC100037680
  • Lmnb1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
5
2
2
1
Methode Type
Sandwich ELISA
Detection Range
0.15-10 ng/mL
Minimum Detection Limit
0.15 ng/mL
Application
ELISA
Options
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Catalog No. ABIN4947815
$ 757.89
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1139986 Log in to see
1 ABIN2949055 0.156-10 ng/mL 0.156 ng/mL Log in to see

Similar Lamin B1 ELISA Kits

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
ELISA 6 ELISA Kits 3 ELISA Kits 3 ELISA Kits

General

Antigen Lamin B1 (LMNB1) ELISA Kits
Reactivity Human, Mouse (Murine), Rat (Rattus)
Kits with alternative reactivity to:
(5), (2), (2), (1)
Methode Type Sandwich ELISA
Detection Range 0.15-10 ng/mL
Minimum Detection Limit 0.15 ng/mL
Application ELISA
Supplier Log in to see

Product Details Lamin B1 ELISA Kit

Target details Application Details Handling Images
Purpose ELISA Kit for Lamin B1 (LMNB1)
Sample Type Cell Lysate, Tissue Homogenate, Biological Fluids
Analytical Method Quantitative
Specificity

This assay has high sensitivity and excellent specificity for detection of Lamin B1 (LMNB1).
No significant cross-reactivity or interference between Lamin B1 (LMNB1) and analogues was observed.

Sensitivity 0.055 ng/mL

Target details

Product Details Lamin B1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Lamin B1 (LMNB1 ELISA Kit Abstract)
Research Area Signaling, Cytoskeleton, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details Lamin B1 ELISA Kit Target details Handling Images back to top
Assay Time 3 h
Plate Pre-coated
Protocol The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin B1 (LMNB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin B1 (LMNB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin B1 (LMNB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lamin B1 (LMNB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Assay Procedure 1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.
Assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lamin B1 (LMNB1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lamin B1 (LMNB1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions For Research Use only

Handling

Product Details Lamin B1 ELISA Kit Target details Application Details Images back to top
Handling Advice

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Storage 4 °C