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|Application / Reactivity||Human|
|Dot Blot (DB)||3 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||8 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||10 Antibodies|
|Immunofluorescence (IF)||36 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||1 Antibodies|
|Immunohistochemistry (IHC)||78 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||57 Antibodies|
|Immunoprecipitation (IP)||12 Antibodies|
|Luminex Assay (LMNX)||10 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Western Blotting (WB)||213 Antibodies|
|Antigen||Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) Antibodies|
|Conjugate||This NME1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|3 references available|
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Product Details anti-NME1 AntibodyTarget Details NME1 Application Details Handling References for anti-NME1 Antibody (ABIN969317) Images
|Immunogen||Purified recombinant fragment of human NME1 expressed in E. coli.|
Target Details NME1Product Details anti-NME1 Antibody Application Details Handling References for anti-NME1 Antibody (ABIN969317) Images back to top
|Alternative Name||NME1 (NME1 Antibody Abstract)|
|Background||Non-metastatic cells 1,protein,with a nm23 nucleoside diphosphate kinase gene family,involved in the phosphorylation of nucleoside diphosphates,with a reduced expression in tumor progression to the metastatic phenotype,mutated in agressive neuroblastoma,expressed in lung carcinoma cell lines not in normal lung,pyrimidine biosynthetic pathway. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. Has tumor metastasis-suppressive capacity.Tissue specificity: Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed.|
|Molecular Weight||17 kDa|
Application DetailsProduct Details anti-NME1 Antibody Target Details NME1 Handling References for anti-NME1 Antibody (ABIN969317) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-NME1 Antibody Target Details NME1 Application Details References for anti-NME1 Antibody (ABIN969317) Images back to top
|Buffer||Ascitic fluid containing 0.03 % sodium azide.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-NME1 Antibody (ABIN969317)Product Details anti-NME1 Antibody Target Details NME1 Application Details Handling Images back to top
|Product cited in:||
Lee, Jeong, Kim et al.: "Multiple functions of Nm23-H1 are regulated by oxido-reduction system." in: PLoS ONE, Vol. 4, Issue 11, pp. e7949, 2009 (PubMed).
Treharne, Giles Best, Mehta: "Transglutaminase 2 and nucleoside diphosphate kinase activity are correlated in epithelial membranes and are abnormal in cystic fibrosis." in: FEBS letters, Vol. 583, Issue 17, pp. 2789-92, 2009 (PubMed).
Egistelli, Chichiarelli, Gaucci et al.: "IFI16 and NM23 bind to a common DNA fragment both in the P53 and the cMYC gene promoters." in: Journal of cellular biochemistry, Vol. 106, Issue 4, pp. 666-72, 2009 (PubMed).
ImagesProduct Details anti-NME1 Antibody Target Details NME1 Application Details Handling References for anti-NME1 Antibody (ABIN969317) back to top