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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||17 Antibodies||19 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||2 Antibodies|
|Immunochromatography (IC)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies||3 Antibodies|
|Immunofluorescence (IF)||14 Antibodies||15 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||22 Antibodies|
|Immunohistochemistry (IHC)||22 Antibodies||20 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||14 Antibodies||13 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies||10 Antibodies|
|Western Blotting (WB)||59 Antibodies||58 Antibodies|
|Antigen||tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, theta Polypeptide (YWHAQ) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This YWHAQ antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-YWHAQ AntibodyTarget Details YWHAQ Application Details Handling Images
|Specificity||This antibody detects endogenous levels of 14-3-3 θ protein. (region surrounding Gln67)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details YWHAQProduct Details anti-YWHAQ Antibody Application Details Handling Images back to top
|Alternative Name||14-3-3 Protein theta (YWHAQ Antibody Abstract)|
|Background||14-3-3 proteins regulate many cellular processes relevant to cancer biology,notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activitiesSynonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ|
|Molecular Weight||approx. 28 kDa|
|Research Area||Transcription Factors|
Application DetailsProduct Details anti-YWHAQ Antibody Target Details YWHAQ Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Images back to top
|Buffer||Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-YWHAQ Antibody Target Details YWHAQ Application Details Handling back to top