14-3-3 theta antibody (N-Term)

Catalog No. ABIN2792144

The Rabbit Polyclonal anti-14-3-3 theta antibody is suitable to detect 14-3-3 theta in samples from Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio) and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for 14-3-3 theta antibody (N-Term) (ABIN2792144)

Target: 14-3-3 theta (YWHAQ)

Reactivity: Human, Mouse, Rat, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-14-3-3 theta Antibody

Binding Specificity: N-Term

Purpose: YWHAQ Antibody - N-terminal region

Sequence: SVAYKNVVGG RRSAWRVISS IEQKTDTSDK KLQLIKDYRE KVESELRSIC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against YWHAQ. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human YWHAQ

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: YWHAQ

Background:

Background Information: YWHAQ belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99 % identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease.

Gene Name: Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide

Alternative Symbols: 1C5, HS1, 14-3-3

Protein Name: 14-3-3 protein theta

Molecular Weight: 28kDa

Gene ID: 10971

NCBI Accession: NP_006817

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction