Accutase® Cell Detachment Solution

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Application
Biochemical Assay (BCA)
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Characteristics Accutase® is a cell detachment solution containing proteolytic and collagenolytic enzymes, which are neither mammalian nor bacterial derived. It is a replacement for Trypsin-EDTA solution and is useful for the detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, and polymers. Accutase® works exceptionally well for detaching cell lines for continued cell culturing, analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, and tumor cell migration assays. Cell lines tested for Accutase™ application include fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and HNK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, MIC5, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251, D54, HT1080 fibrosarcoma cells, HT29 colon cancer cells, Sf9 insect cells, human embryonic stem cells, human mesenchymal stem cells, and human neural stem cells.
Components Accutase Cell Detachment Solution
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer Accutase® enzymes in Dulbecco's PBS containing 0.5 mM EDTA and phenol red.
Storage -20 °C
Storage Comment Upon receipt, store at -20°C. It can also be stored between 2°C and 8°C for up to 2 months.
Expiry Date 2 months
Supplier Images
Flow Cytometry (FACS) image for Accutase® Cell Detachment Solution (ABIN2669167) Accutase® Cell Detachment Solution
Flow Cytometry (FACS) image for Accutase® Cell Detachment Solution (ABIN2669167) Accutase® Cell Detachment Solution (Image 2)
Background publications Bajpai, Lesperance, Kim, Terskikh: "Efficient propagation of single cells Accutase-dissociated human embryonic stem cells." in: Molecular reproduction and development, Vol. 75, Issue 5, pp. 818-27, 2008 (PubMed).

Yang, Zhang, Oldenburg, Ayala, Zhang: "Human embryonic stem cell-derived dopaminergic neurons reverse functional deficit in parkinsonian rats." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 1, pp. 55-63, 2008 (PubMed).

Brevig, Røhrmann, Riemann: "Oxygen reduces accumulation of type IV collagen in endothelial cell subcellular matrix via oxidative stress." in: Artificial organs, Vol. 30, Issue 12, pp. 915-21, 2006 (PubMed).