anti-Human RAF1 antibody for ELISA

Recommended RAF1 Antibody (supplied by: Log in to see )

Antigen
V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
  • c-raf
  • craf
  • raf1
  • RAF1
  • raf
  • CRAF
  • NS5
  • Raf-1
  • c-Raf
  • 6430402F14Rik
  • AA990557
  • BB129353
  • Craf1
  • D830050J10Rik
  • v-Raf
  • AFR1
  • RAF
  • Afr-1
  • Afr1
  • Raf
  • mKIAA0854
  • v-raf-1 murine leukemia viral oncogene homolog 1a
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • raf protein
  • v-raf-leukemia viral oncogene 1
  • zinc fingers and homeoboxes 2
  • raf1a
  • RAF1
  • raf1
  • LOC100341921
  • LOC100347407
  • c-raf
  • Raf1
  • ZHX2
  • Zhx2
Reactivity
Human
834
412
387
25
23
16
16
13
8
8
8
5
4
4
2
2
1
1
1
Host
Mouse
780
62
12
6
2
Clonality
Monoclonal
Conjugate
This RAF1 antibody is un-conjugated
45
45
44
30
29
29
14
14
12
12
12
12
12
12
12
12
12
2
2
2
2
2
2
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
512
364
220
172
156
104
52
48
20
18
18
7
2
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN969560
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.482866 ABIN969559 FACS IHC ELISA WB Mouse IgG1 Log in to see 4G4 2
12.482866 ABIN4349225 CyTOF ELISA FACS IHC WB Mouse IgG1 Log in to see 4G4
12.482866 ABIN1846392 FACS IHC ELISA WB Mouse IgG1 Log in to see
12.482866 ABIN1844891 FACS IHC ELISA WB Mouse IgG1 Log in to see
12.482866 ABIN1847644 IHC ELISA Rabbit Internal Region, pThr269 Log in to see Polyclonal
1 ABIN1531571 ELISA WB Rabbit IgG pSer43 Log in to see Polyclonal 2
1 ABIN1531958 IHC ELISA WB Rabbit IgG pSer259 Log in to see Polyclonal 1
1 ABIN1532076 IHC ELISA Rabbit IgG pThr269 Log in to see Polyclonal 1
1 ABIN1532077 IHC ELISA Rabbit IgG pSer289 Log in to see Polyclonal 1
1 ABIN1532586 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1532992 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1533109 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
1 ABIN1531386 IF IHC ELISA WB Rabbit IgG pSer621 Log in to see Polyclonal
1 ABIN1531428 IHC ELISA WB Rabbit IgG pSer296 Log in to see Polyclonal
1 ABIN1531959 ELISA WB Rabbit IgG pSer338 Log in to see Polyclonal
1 ABIN1531385 IHC ELISA WB Rabbit IgG pTyr341 Log in to see Polyclonal
1 ABIN1532404 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1532405 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1532444 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1532993 ELISA WB Rabbit IgG Log in to see Polyclonal

General

Antigen V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) Antibodies
Reactivity Human
(834), (412), (387), (25), (23), (16), (16), (13), (8), (8), (8), (5), (4), (4), (2), (2), (1), (1), (1)
Host Mouse
(780), (62), (12), (6), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RAF1 antibody is un-conjugated
(45), (45), (44), (30), (29), (29), (14), (14), (12), (12), (12), (12), (12), (12), (12), (12), (12), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(512), (364), (220), (172), (156), (104), (52), (48), (20), (18), (18), (7), (2), (2), (2), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-RAF1 Antibody

Target Details RAF1 Application Details Handling ProductDetails: References for anti-RAF1 antibody (ABIN969560) Images
Purification Purified
Immunogen Purified recombinant fragment of human RAF1 expressed in E. Coli.
Clone 4G4
Isotype IgG1

Target Details RAF1

Product Details anti-RAF1 Antibody Application Details Handling ProductDetails: References for anti-RAF1 antibody (ABIN969560) Images back to top
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Molecular Weight 73 kDa
Gene ID 5894
HGNC 5894
Research Area Signaling, Kinases/Phosphatases
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-RAF1 Antibody Target Details RAF1 Handling ProductDetails: References for anti-RAF1 antibody (ABIN969560) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details ProductDetails: References for anti-RAF1 antibody (ABIN969560) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

ProductDetails: References for anti-RAF1 antibody (ABIN969560)

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling Images back to top
Product cited in:

Takezawa, Okamoto, Yonesaka, Hatashita, Yamada, Fukuoka, Nakagawa: "Sorafenib inhibits non-small cell lung cancer cell growth by targeting B-RAF in KRAS wild-type cells and C-RAF in KRAS mutant cells." in: Cancer research, Vol. 69, Issue 16, pp. 6515-21, 2009

Peng, Guo, Ji, Ren, Yang, Li, Qu, Li, Tang, Yan, Tang: "Sorafenib induces growth inhibition and apoptosis in human synovial sarcoma cells via inhibiting the RAF/MEK/ERK signaling pathway." in: Cancer biology & therapy, Vol. 8, Issue 18, pp. 1729-36, 2009

Images

Product Details anti-RAF1 Antibody Target Details RAF1 Application Details Handling ProductDetails: References for anti-RAF1 antibody (ABIN969560) back to top
Supplier Images
ELISA image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 2: Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and ne...
Immunohistochemistry (IHC) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues us...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 4: Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG...
Western Blotting (WB) image for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN969560) Figure 5: Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recom...