anti-Rat (Rattus) TJP1 antibody for ELISA

Recommended TJP1 Antibody (supplied by: Log in to see )

Antigen
Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antibodies
  • ZO-1
  • ZO1
  • zo1
  • tight junction protein 1
  • tight junction protein 1 (zona occludens 1)
  • zonula occludens 1
  • TJP1
  • Tjp1
  • ZO1
Epitope
AA 463-1109
16
10
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
92
32
32
20
10
4
4
4
3
2
1
1
1
1
Host
Rabbit
74
16
3
3
2
Clonality
Polyclonal
Conjugate
This TJP1 antibody is un-conjugated
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
74
25
24
23
22
14
13
11
10
7
7
3
2
1
1
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Catalog No. ABIN305736
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2902699 ELISA IF/ICC IHC WB Rabbit AA 23-502 Log in to see Polyclonal

General

Antigen Tight Junction Protein 1 (Zona Occludens 1) (TJP1) Antibodies
Epitope AA 463-1109
(16), (10), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)
(92), (32), (32), (20), (10), (4), (4), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(74), (16), (3), (3), (2)
Clonality
Polyclonal
Conjugate This TJP1 antibody is un-conjugated
(3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(74), (25), (24), (23), (22), (14), (13), (11), (10), (7), (7), (3), (2), (1), (1)
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Product Details anti-TJP1 Antibody

Target Details TJP1 Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.
Purification Immunoaffinity purified
Immunogen A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.

Type of Immunogen: Fusion protein
Isotype IgG

Target Details TJP1

Product Details anti-TJP1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TJP1 / ZO-1 (TJP1 Antibody Abstract)
Background Name/Gene ID: TJP1
Family: Occludin

Synonyms: TJP1, ZO-1, Zona occludens protein 1, Zona occludens 1, Zonula occludens protein 1, Tight junction protein 1, ZO1, Tight junction protein ZO-1, Zonula occludens 1 protein
Gene ID 7082
UniProt Q07157
Pathways Carbohydrate Homeostasis, Cell-Cell Junction Organization

Application Details

Product Details anti-TJP1 Antibody Target Details TJP1 Handling Images back to top
Application Notes Approved: ELISA, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Immunoprecipitation: 2-5 μg/reaction.
Restrictions For Research Use only

Handling

Product Details anti-TJP1 Antibody Target Details TJP1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.4, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-TJP1 Antibody Target Details TJP1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 463-1109) antibody (ABIN305736) Human Kidney (formalin-fixed, paraffin-embedded) stained with TJP1 antibody ABIN30573...