anti-Human C1S antibody for Inhibition Assay

Recommended C1S Antibody (supplied by: Log in to see )

Antigen
Complement Component 1, S Subcomponent (C1S) Antibodies
  • C1S
  • r-gsp
  • AA959438
  • AI255193
  • AI327365
  • C1sa
  • complement component 1, s subcomponent
  • C1S
  • c1s
  • LOC100222051
  • C1s
Reactivity
Human
80
21
21
3
1
Host
Mouse
46
30
4
Clonality
Monoclonal
Conjugate
This C1S antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
50
26
24
13
13
10
7
5
3
3
3
2
2
1
1
1
1
1
1
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Catalog No. ABIN2191986
$ 509.36
Plus shipping costs $45.00

General

Antigen Complement Component 1, S Subcomponent (C1S) Antibodies
Reactivity Human
(80), (21), (21), (3), (1)
Host Mouse
(46), (30), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C1S antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Inhibition Assay (InhA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(50), (26), (24), (13), (13), (10), (7), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-C1S Antibody

Target Details C1S Application Details Handling References for anti-C1S antibody (ABIN2191986) Images
Sterility 0.2 μm filtered
Clone M81

Target Details C1S

Product Details anti-C1S Antibody Application Details Handling References for anti-C1S antibody (ABIN2191986) Images back to top
Antigen
Alternative Name c1s (C1S Antibody Abstract)
Background Monoclonal antibody M81 reacts with an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). Activated C1s is a glycosylated single-polypeptide zymogen, MW 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognised by the antibody M81 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M81 blocks C4 activation and C4 binding to activated C1s. The antibody reacts around the binding site of C1s and reacts with both active and inactive C1s.
Pathways Complement System

Application Details

Product Details anti-C1S Antibody Target Details C1S Handling References for anti-C1S antibody (ABIN2191986) Images back to top
Application Notes For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of C4 activation by C1s dilutions have to be made according to the amounts C1s to be inactivated.
Restrictions For Research Use only

Handling

Product Details anti-C1S Antibody Target Details C1S Application Details References for anti-C1S antibody (ABIN2191986) Images back to top
Buffer PBS containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-C1S antibody (ABIN2191986)

Product Details anti-C1S Antibody Target Details C1S Application Details Handling Images back to top
Product cited in:

Matsumoto, Nagaki, Kitamura, Kuramitsu, Nagasawa, Seya: "Probing the C4-binding site on C1s with monoclonal antibodies. Evidence for a C4/C4b-binding site on the gamma-domain." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2743-50, 1989