anti-Guinea Pig C3 antibody for Western Blotting

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Guinea Pig, Human, Rabbit
281
78
64
16
8
7
7
4
3
1
1
1
Host
Mouse
139
120
103
27
15
9
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
33
31
21
6
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
213
196
74
72
67
57
56
50
44
39
38
32
11
5
5
4
3
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN111064
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN568552 DB EIA ID IF IP WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN568553 DB EIA ID IF IP WB FITC Goat IgG Log in to see Polyclonal
1 ABIN568554 DB EIA ID IF IP WB HRP Goat IgG Log in to see Polyclonal
1 ABIN215710 IHC (fro) WB Mouse IgG2b Log in to see H13
1 ABIN5573358 ELISA IHC WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN5573361 ELISA IHC WB HRP Goat IgG Log in to see Polyclonal
1 ABIN5573354 ELISA IP WB Goat IgG Log in to see Polyclonal

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Guinea Pig, Human, Rabbit
(281), (78), (64), (16), (8), (7), (7), (4), (3), (1), (1), (1)
Host Mouse
(139), (120), (103), (27), (15), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(33), (31), (21), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(213), (196), (74), (72), (67), (57), (56), (50), (44), (39), (38), (32), (11), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 antibody (ABIN111064) Images
Specificity This Monoclonal antibody Clone H 13 recognizes a C3a-determinant (eg C3 alpha-chain) which is present also on native C3. H 13 allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment, C3b or C are negative with H 13.
Cross-Reactivity (Details) Species reactivity (tested):Human, Guinea Pig and Rabbit.
Purification Protein A Chromatography
Immunogen Human complement component C3
Clone H13
Isotype IgG2b

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 antibody (ABIN111064) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
Gene ID 718
NCBI Accession NP_000055
UniProt P01024
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 antibody (ABIN111064) Images back to top
Application Notes Immunohistochemistry on frozen sections: 1: 10. Western Blot. ELISA. Detection of C3 deposits in tissue, cells, micro-organisms and immune complexes. Itshould be used with positive control anti-C3b (H11/H206). Non-activated, absorbed C3contains the C3a determinant, whereas activated C3 is negative with H 13
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 antibody (ABIN111064) Images back to top
Reconstitution Restore with 1 mL distilled water
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-C3 antibody (ABIN111064)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Background publications

Zilow, Naser, Rutz, Burger: "Quantitation of the anaphylatoxin C3a in the presence of C3 by a novel sandwich ELISA using monoclonal antibody to a C3a neoepitope." in: Journal of immunological methods, Vol. 121, Issue 2, pp. 261-8, 1989

Burger, Bader, Kirschfink, Rother, Schrod, Wörner, Zilow: "Functional analysis and quantification of the complement C3 derived anaphylatoxin C3a with a monoclonal antibody." in: Clinical and experimental immunology, Vol. 68, Issue 3, pp. 703-11, 1987

Sugita, Majdic, Stockinger, Holter, Burger, Knapp: "Induction of human complement activation without cytolysis by mouse monoclonal antibodies to human leukocyte antigens." in: Transplantation, Vol. 43, Issue 4, pp. 570-4, 1987