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anti-Human C3 antibody for Dot Blot

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Human
264
73
61
15
7
6
5
4
4
1
1
1
Host
Goat
134
111
90
25
14
8
Clonality
Polyclonal
Conjugate
This C3 antibody is conjugated to HRP
31
29
17
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
193
182
70
64
60
51
46
41
41
39
34
24
11
4
4
3
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN568572
$ 404.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN568570 DB EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal

Similar anti-C3 Antibodies

Application / Reactivity Human
Dot Blot (DB) 2 Antibodies
ELISA 124 Antibodies
Enzyme Immunoassay (EIA) 28 Antibodies
Flow Cytometry (FACS) 26 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 5 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunodiffusion (ID) 12 Antibodies
Immunoelectrophoresis (IEP) 1 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 34 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Human
(264), (73), (61), (15), (7), (6), (5), (4), (4), (1), (1), (1)
Host Goat
(134), (111), (90), (25), (14), (8)
Clonality
Polyclonal
Conjugate This C3 antibody is conjugated to HRP
(31), (29), (17), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Dot Blot (DB), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(193), (182), (70), (64), (60), (51), (46), (41), (41), (39), (34), (24), (11), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity In Immunoelectrophoresis against fresh Human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Characteristics Molar Ratio: Peroxidase/IgG ~1.7
Purification The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.
Immunogen C3c is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods. Freund’s complete adjuvant is used in the first step of the immunization.
Isotype IgG

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chainSynonyms: CPAMD1, Complement component 3
Gene ID 718
NCBI Accession NP_000055
UniProt P01024
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Reconstitution Restore by adding 1 mL of sterile distilled water.
Concentration 10 mg/mL
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid Repeated thawing and freezing.
Storage 4 °C/-20 °C
Storage Comment Store lyophilized at 2-8 °C and reconstituted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.