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anti-Mouse (Murine) C3 antibody for Enzyme Immunoassay

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • AI255234
  • ARMD9
  • asp
  • ASP
  • c3
  • C3
  • C3-1
  • C3a
  • C3b
  • C3d
  • c3_D
  • CPAMD1
  • cpamd1
  • HSE-MSF
  • Plp
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
Reactivity
Mouse (Murine)
237
48
36
13
5
5
5
3
2
1
Host
Rat
102
100
84
13
7
7
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
31
29
17
3
3
3
3
3
3
3
3
3
2
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
153
150
54
42
42
35
33
30
28
25
23
8
5
4
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN114403
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008899989 ABIN783940 EIA ID IHC (fro) IF IP WB HRP Goat IgG Log in to see Polyclonal
0.0008899989 ABIN783938 EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal
0.0008899989 ABIN783937 EIA ID IHC (fro) IF IP Goat Log in to see Polyclonal

Top referenced anti-C3 antibody for Enzyme Immunoassay

  • Mouse (Murine) Monoclonal C3 Primary Antibody for EIA, FACS - ABIN114403 (2 Publications): Mastellos, Prechl, László, Papp, Oláh, Argyropoulos, Franchini, Tudoran, Markiewski, Lambris, Erdei: Novel monoclonal antibodies against mouse C3 interfering with complement activation: description of fine specificity and applications to various immunoassays. in Molecular immunology 2004 (PubMed)

Similar anti-C3 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 13 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Western Blotting (WB) 26 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Mouse (Murine)
(237), (48), (36), (13), (5), (5), (5), (3), (2), (1)
Host Rat
(102), (100), (84), (13), (7), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(31), (29), (17), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(153), (150), (54), (42), (42), (35), (33), (30), (28), (25), (23), (8), (5), (4), (3), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 Antibody (ABIN114403) Images
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Protein G Chromatography.
Immunogen C57BL/6 thymocytes incubated with a rat IgG2b anti-murine Thy-1 (RmT1) and C57BL/6 serum (as a source for C1q). Donor: Lou/c rat spleen cells. Fusion Partner: Mouse myeloma cell line P3X63 Ag. 8.653.
Clone RMC11H9
Isotype IgG2a

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 Antibody (ABIN114403) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3 is the most abundant complement protein in serum. C3 and its cleavage products, C3a and C3b, play a central role in the complement activation cascade. C3b forms an integral part of the C3 and C5 convertases as it promotes complement activation and the subsequent formation of the membrane attack complex. C3a possesses anaphlatoxic as well as various immunoregulatory properties. Also, C3 has been implicated in developmental and non-imflammatory process such as hematopoiesis, skeletal and vascular development and reproduction.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
Gene ID 10090
UniProt P01027
Research Area Complement Factors
Pathways Complement System

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 Antibody (ABIN114403) Images back to top
Application Notes ELISA. Western blot. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Acetone Fixed Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium . 2. Wash 2 times. 3. Resuspend cells to 1x10e6 cells in approximately 50 µl Media A in a microcentrifugetube, i. e. 50 µl of cells resuspended to 2x10e7 cells/ml (the contents of 1 tube = 1 test). 4. To each tube add 0. 5 µg of ABIN114403 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at appropriate dilution. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. (This stains dead cells by intercalating DNA). Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 Antibody (ABIN114403) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-C3 Antibody (ABIN114403)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Background publications

Mastellos, Prechl, László, Papp, Oláh, Argyropoulos, Franchini, Tudoran, Markiewski, Lambris, Erdei: "Novel monoclonal antibodies against mouse C3 interfering with complement activation: description of fine specificity and applications to various immunoassays." in: Molecular immunology, Vol. 40, Issue 16, pp. 1213-21, 2004

Kremmer, Thierfelder, Felber, Hoffmann-Fezer, Wasiliu: "Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4." in: Hybridoma, Vol. 9, Issue 4, pp. 309-17, 1990

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling References for anti-C3 Antibody (ABIN114403) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-C3 antibody (Complement Component 3) (ABIN114403) Representative histogram of thymocytes stained with ABIN114403-FITC label