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anti-Human Complement Factor H antibody for Radial Immunodiffusion

Recommended Complement Factor H Antibody (supplied by: Log in to see )

Antigen
Complement Factor H (CFH) Antibodies
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFH
  • CFHL3
  • fH
  • Fh
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • im:7147412
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • si:ch211-207o17.2
Reactivity
Human
204
30
24
3
3
2
2
1
1
Host
Sheep
93
71
31
26
2
1
Clonality
Polyclonal
Conjugate
This Complement Factor H antibody is un-conjugated
17
11
5
3
3
3
2
2
2
2
2
2
2
2
2
Application
Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
139
124
43
39
31
22
21
18
12
11
9
6
5
4
1
1
1
1
1
1
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Catalog No. ABIN237215
$ 412.13
Plus shipping costs $45.00

Similar anti-Complement Factor H Antibodies

Application / Reactivity Human
ELISA 118 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Immunoassay (IA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunodiffusion (ID) 14 Antibodies
Immunoelectrophoresis (IEP) 2 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 45 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunoprecipitation (IP) 31 Antibodies

General

Antigen Complement Factor H (CFH) Antibodies
Reactivity Human
(204), (30), (24), (3), (3), (2), (2), (1), (1)
Host Sheep
(93), (71), (31), (26), (2), (1)
Clonality
Polyclonal
Conjugate This Complement Factor H antibody is un-conjugated
(17), (11), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)
(139), (124), (43), (39), (31), (22), (21), (18), (12), (11), (9), (6), (5), (4), (1), (1), (1), (1), (1), (1)
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Product Details anti-Complement Factor H Antibody

Target Details Complement Factor H Application Details Handling Images
Specificity Gives a single arc when tested by IEP against human plasma. Identity is confirmed by double diffusion (Ouchterlony) against human plasma and a known anti-human Factor H. The cross reactivity of this product against the sera of the species listed below has been assessed by double diffusion using 10ul of antisera against 10ul of species sera. These results are intended as a guideline only. It is possible that cross-reactivity against species listed as negative above may be observed when using different test conditions or techniques.
Characteristics Sheep anti Factor H (Beta 1H), Sheep antibody to Human Factor H (Beta 1H)
Purification Delipidated and if necessary, absorbed to monospecificity by use of solid-phase absorbants. An immunoglobulin fraction is then produced. The titer is adjusted so that inter-batch variation is within 10 %.
Sterility 0.2 μm filtered
Immunogen Human 155 kDa Factor H purified from plasma, shown to be pure by IEP

Target Details Complement Factor H

Product Details anti-Complement Factor H Antibody Application Details Handling Images back to top
Antigen
Alternative Name Factor H (Beta 1H) (CFH Antibody Abstract)
Pathways Complement System

Application Details

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Handling Images back to top
Application Notes Suitable for use in a variety of gel techniques including radial immunodiffusion (RID), double diffusion, immunoelectrophoresis (IEP). The use of 3 % PEG 6000 with 1.3 % agarose in a suitable buffer (such as TBE or tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, enzyme-linked immunosorbent assays and Western blot and IHC has not been assessed but use in such assays should not necessarily be excluded. Each laboratory should determine an optimum working titer for use in its particular application. RID : 1 µL antiserum/cm2 gel vs. 1 µL neat - 1:10 dilution human plasma. Double Diffusion: 10 µL antiserum vs. 10 µL plasma. IEP: 101 µL antiserum vs. 1 µL plasma.
Restrictions For Research Use only

Handling

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Images back to top
Format Liquid
Buffer Glycine buffered saline, pH 7.4, 0.1 % Sodium azide, 0.1 % EACA, 0.01 % Benzamidine, 1 mM EDTA
Preservative Sodium azide, EACA, Benzamidine
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Slight precipitation can occur on storage which may be removed by centrifugation, and should not affect performance characteristics.
Prepare working dilution only prior to immediate use.
Storage 4 °C
Storage Comment Upon receipt, store at 2-8 °C.