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Human Kallikrein 5 ELISA Kit

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Antigen
Kallikrein 5 (KLK5) ELISA Kits
  • 1110030O19Rik
  • KAL
  • KALA
  • KLK-L2
  • Klk1
  • Klk5
  • KLKL2
  • RATKALA
  • SCTE
Epitope
AA 23-293
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
14
5
3
2
2
2
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
78-5000 pg/mL
Minimum Detection Limit
78 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1672807
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414986 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Saliva, Biological Fluids 31.25-2000 pg/mL 31.25 pg/mL Log in to see
0.32693866 ABIN2708301 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 78-5000 pg/mL 78 pg/mL Log in to see
0.32693866 ABIN2114380 Cell Culture Supernatant, Serum, Plasma (EDTA - heparin), Milk, Saliva Log in to see
0.0012117105 ABIN842255 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Saliva 62.5-4000 pg/mL 62.5 pg/mL Log in to see
0.0012117105 ABIN1570923 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 78.125-5000 pg/mL 78.125 pg/mL Log in to see
0.0012117105 ABIN1503362 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
0.0012117105 ABIN2042572 Sandwich ELISA 8-5000 pg/mL 8 pg/mL Log in to see
0.0012117105 ABIN2684807 Sandwich ELISA Cell Culture Supernatant, Body Fluids, Plasma, Serum, Tissue Lysate Log in to see
0.0012117105 ABIN2535761 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Saliva, Biological Fluids 78.12-5000 pg/mL 78.12 pg/mL Log in to see
0.0012117105 ABIN454379 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN2089411 Sandwich ELISA Log in to see
0.0012117105 ABIN2116176 78-5000 pg/mL 78 pg/mL Log in to see
0.0012117105 ABIN2948956 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN2112645 Competition ELISA Log in to see

Top referenced Kallikrein 5 ELISA Kit for ELISA

Similar Kallikrein 5 ELISA Kits

Application / Reactivity Human
ELISA 15 ELISA Kits

General

Antigen Kallikrein 5 (KLK5) ELISA Kits
Epitope AA 23-293
Reactivity Human
Kits with alternative reactivity to:
(14), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 78-5000 pg/mL
Minimum Detection Limit 78 pg/mL
Application ELISA
Pubmed 1 reference available
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Product Details Kallikrein 5 ELISA Kit

Target details Application Details Handling References for Kallikrein 5 Kit (ABIN1672807) Images
Purpose For quantitative detection of human KLK5 in cell culture supernates, serum, plasma(heparin , EDTA), human milk and saliva.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk, Saliva
Detection Method Colorimetric
Specificity Natural and recombinant human KLK5
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human KLK5 antibod - 1
  • Lyophilized recombinant human KLK5 standar - 10ng/tubex2
  • Biotinylated anti- human KLK5 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: V23-S293

Target details

Product Details Kallikrein 5 ELISA Kit Application Details Handling References for Kallikrein 5 Kit (ABIN1672807) Images back to top
Antigen
Alternative Name KLK5 (KLK5 ELISA Kit Abstract)
Background Kallikrein gene 5(KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding(desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.
Gene ID 25818
UniProt Q9Y337
Pathways Complement System

Application Details

Product Details Kallikrein 5 ELISA Kit Target details Handling References for Kallikrein 5 Kit (ABIN1672807) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human KLK5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK5 has been precoated onto 96-well plates. Standards(NSO, V23-S293) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KLK5 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human KLK5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), human milk or saliva to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human KLK5 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 545, Standard deviation: 38.7, CV(%): 7.1
  • Sample 2: n=16, Mean(pg/ml): 1823, Standard deviation: 69.3, CV(%): 3.8
  • Sample 3: n=16, Mean(pg/ml): 3240, Standard deviation: 158.8, CV(%): 4.9,
  • Sample 1: n=24, Mean(pg/ml): 613, Standard deviation: 46.6, CV(%): 7.6
  • Sample 2: n=24, Mean(pg/ml): 1972, Standard deviation: 98.6, CV(%): 5
  • Sample 3: n=24, Mean(pg/ml): 3468, Standard deviation: 260.1, CV(%): 7.5
Restrictions For Research Use only

Handling

Product Details Kallikrein 5 ELISA Kit Target details Application Details References for Kallikrein 5 Kit (ABIN1672807) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Kallikrein 5 Kit (ABIN1672807)

Product Details Kallikrein 5 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Kim, Scorilas, Katsaros, Yousef, Massobrio, Fracchioli, Piccinno, Gordini, Diamandis: "Human kallikrein gene 5 (KLK5) expression is an indicator of poor prognosis in ovarian cancer." in: British journal of cancer, Vol. 84, Issue 5, pp. 643-50, 2001

Images

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Supplier Images
ELISA image for Kallikrein 5 ELISA Kit (KLK5) (ABIN1672807) Human KLK5 PicoKine ELISA Kit standard curve