Mouse (Murine) MBL2 ELISA Kit

Recommended MBL2 Product (supplied by: Log in to see )

Antigen
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA Kits
  • MBP-C
  • mbl2
  • MBL
  • Ab2-001
  • Ab2-011
  • L-MBP
  • MBL-C
  • COLEC1
  • HSMBPC
  • MBL2D
  • MBP
  • MBP1
  • MBPD
  • pMBP-27
  • MBL1
  • cMBl
  • collectin
  • mannose-binding lectin (protein C) 2, soluble
  • Mannose-binding protein C
  • mannose-binding lectin (protein C) 2, soluble (opsonic defect)
  • mannose-binding lectin (protein C) 2
  • MBL2
  • mbl2
  • Mbl2
Epitope
AA 19-244
Reactivity
Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
11
9
9
4
2
1
1
Method Type
Sandwich ELISA
Detection Range
312-20000 pg/mL
Minimum Detection Limit
312 pg/mL
Application
ELISA
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2859303
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1571155 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN817832 Sandwich ELISA Serum, Plasma, Tissue Homogenate 20-0.31 ng/mL 20 ng/mL Log in to see
1 ABIN5525094 Sandwich ELISA Plasma, Serum, Biological Fluids, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN4885612 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate Log in to see
1 ABIN2684938 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum 312-20000 pg/mL 312 pg/mL Log in to see
1 ABIN2684937 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
1 ABIN2536367 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN4970979 Sandwich ELISA Cell Culture Supernatant, Plasma, Tissue Homogenate, Serum, Cell Lysate, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN455818 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN2949329 78-5000 pg/mL 78 pg/mL Log in to see
1 ABIN5073278 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

Similar MBL2 ELISA Kits

Application / Reactivity Chicken Cow (Bovine) Human Pig (Porcine) Rat (Rattus) Sheep (Ovine)
ELISA 4 ELISA Kits 1 ELISA Kits 9 ELISA Kits 2 ELISA Kits 9 ELISA Kits 1 ELISA Kits

General

Antigen Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA Kits
Epitope AA 19-244
Reactivity Mouse (Murine)
Kits with alternative reactivity to:
(11), (9), (9), (4), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 312-20000 pg/mL
Minimum Detection Limit 312 pg/mL
Application ELISA
Supplier Log in to see

Product Details MBL2 ELISA Kit

Target details Application Details Handling Images
Purpose Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MBL2
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Analytical Method Quantitative
Specificity Expression system for standard: NSO
Immunogen sequence: E19-D244
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: E19-D244

Target details

Product Details MBL2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name MBL2 (MBL2 ELISA Kit Abstract)
Background

Protein Function: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity). .

Background: MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Synonyms: Mannose-binding protein C,MBP-C,Mannan-binding protein,RA-reactive factor P28A subunit,RARF/P28A,Mbl2,

Full Gene Name: Mannose-binding protein C

Cellular Localisation: Secreted.
Gene ID 17195
UniProt P41317
Pathways Complement System, Positive Regulation of Immune Effector Process

Application Details

Product Details MBL2 ELISA Kit Target details Handling Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Comment

Sequence similarities: Contains 1 C-type lectin domain.

Plate Pre-coated
Protocol mouse MBL2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MBL2 has been precoated onto 96-well plates. Standards(NSO, E19-D244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MBL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MBL2 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse MBL2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MBL2 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 3.2, Standard deviation: 0.157, CV(%): 4.9
  • Sample 2: n=16, Mean(ng/ml): 8.4, Standard deviation: 0.378, CV(%): 4.5
  • Sample 3: n=16, Mean(ng/ml): 12.8, Standard deviation: 0.474, CV(%): 3.7,
  • Sample 1: n=24, Mean(ng/ml): 3.6, Standard deviation: 0.23, CV(%): 6.4
  • Sample 2: n=24, Mean(ng/ml): 8.9, Standard deviation: 0.516, CV(%): 5.8
  • Sample 3: n=24, Mean(ng/ml): 13.1, Standard deviation: 0.707, CV(%): 5.4
Restrictions For Research Use only

Handling

Product Details MBL2 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid multiple freeze-thaw cycles.
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details MBL2 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for MBL2 ELISA Kit (Mannose-Binding Lectin (Protein C) 2, Soluble) (ABIN2859303) Mouse MBL2 PicoKine ELISA Kit standard curve