KLRG1 Protein (AA 60-195) (Fc Tag)
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- Target See all KLRG1 Proteins
- KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 60-195
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This KLRG1 protein is labelled with Fc Tag.
- Purpose
- Human KLRG1 Protein
- Sequence
- Leu60-Phe195
- Characteristics
- Recombinant Human KLRG1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu60-Phe195.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human KLRG1, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-KLRG1 Antibody, hFc Tag with the EC50 of 18.2ng/ml determined by ELISA. See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))
- Alternative Name
- KLRG1 (KLRG1 Products)
- Synonyms
- KLRG1 Protein, 2F1-Ag Protein, MAFA Protein, MAFA-L Protein, 2F1 Protein, CLEC15A Protein, MAFA-2F1 Protein, MAFA-LIKE Protein, killer cell lectin like receptor G1 Protein, killer cell lectin-like receptor subfamily G, member 1 Protein, KLRG1 Protein, Klrg1 Protein
- Background
- Immune homeostasis requires the tight, tissue-specific control of the different CD4 Foxp3 regulatory T (Treg) cell populations. The cadherin-binding inhibitory receptor killer cell lectin-like receptor G1 (KLRG1) is expressed by a subpopulation of Treg cells with GATA3 effector phenotype.Lack of KLRG1 on Treg cells conferred on them a competitive advantage in the gut, but not in lymphoid organs. Hence, although absence of KLRG1 is not enough to increase intestinal Treg cells in KLRG1 knockout mice, KLRG1 ligation reduces T-cell receptor signals and the competitive fitness of individual Treg cells in the intestine.
- Molecular Weight
- 42.8 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
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