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CHRNA3 antibody (AA 32-240)

Name: CHRNA3 antibody (AA 32-240)
Brand: antibodies-online
SKU: ABIN5611357
Price: 511.5 USD
Availability: InStock

CHRNA3 Reactivity: Human WB, ELISA, IHC, ICC, FACS, Neut Host: Mouse Monoclonal 6D3A10 unconjugated

Western blot analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (CHRNA3 antibody (AA 32-240))

Flow cytometric analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red). (CHRNA3 antibody (AA 32-240))

FACS

Immunofluorescence analysis of Hela cells using CHRNA3 mouse mAb (green). (CHRNA3 antibody (AA 32-240))

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng) (CHRNA3 antibody (AA 32-240))

ELISA

Immunofluorescence analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). (CHRNA3 antibody (AA 32-240))

Western blot analysis using CHRNA3 mAb against HEK293 (1) and CHRNA3 (AA: 32-240)-hIgGFc transfected HEK293 (2) cell lysate. (CHRNA3 antibody (AA 32-240))

Flow cytometric analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red). (CHRNA3 antibody (AA 32-240))

FACS

7 images

(1 reference)

Catalog No. ABIN5611357

Datasheet as PDF

Target See all CHRNA3 Antibodies

CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))
Binding Specificity
15

9

8

6

2

2

1

1

1

1

1
AA 32-240
Reactivity
57

19

18

5

5

4

3

3

2

1

1

1
Human
Host
56

1
Mouse
Compatible Secondaries
Clonality
56

1
Monoclonal
Conjugate
25

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CHRNA3 antibody is un-conjugated
Application
48

21

20

13

13

7

4

4

4

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Neutralization (Neut)

Purification

purified

Immunogen

Purified recombinant fragment of human CHRNA3 (AA: 32-240) expressed in E. coli.

Clone

6D3A10

Isotype

IgG1
anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 32-240) antibody
CHRNA3 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 25-52), (N-Term) antibody
CHRNA3 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB25608 unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 26-75) antibody
CHRNA3 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Horse, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) antibody
CHRNA3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (Center) antibody
CHRNA3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (AA 35-84) antibody
CHRNA3 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Monkey, Pig WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) (N-Term) antibody
CHRNA3 Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig WB Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) antibody
CHRNA3 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) antibody
CHRNA3 Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3) antibody
CHRNA3 Reactivity: Human, Mouse, Rat WB, FACS Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400, IHC: N/A

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Kulbatskii, Shenkarev, Bychkov, Loktyushov, Shulepko, Koshelev, Povarov, Popov, Peigneur, Chugunov, Kozlov, Sharonova, Efremov, Skrebitsky, Tytgat, Kirpichnikov, Lyukmanova: "Human Three-Finger Protein Lypd6 Is a Negative Modulator of the Cholinergic System in the Brain." in: Frontiers in cell and developmental biology, Vol. 9, pp. 662227, (2021) (PubMed).
Target

CHRNA3 (Cholinergic Receptor, Nicotinic, alpha 3 (Neuronal) (CHRNA3))

Alternative Name

CHRNA3 (CHRNA3 Products)

Synonyms

LNCR2 antibody, NACHRA3 antibody, PAOD2 antibody, (a)3 antibody, A730007P14Rik antibody, Acra-3 antibody, Acra3 antibody, cholinergic receptor nicotinic alpha 3 subunit antibody, cholinergic receptor, nicotinic, alpha polypeptide 3 antibody, CHRNA3 antibody, Chrna3 antibody

Background

Description: This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
Aliases: LNCR2, PAOD2, NACHRA3

Molecular Weight

57.5 kDa

Gene ID

1136

HGNC

1136

Pathways

Synaptic Membrane