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UNC13A/Munc13-1 antibody (AA 3-317)

UNC13A Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio) WB, IP, ICC, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1742277
  • Target See all UNC13A/Munc13-1 (UNC13A) Antibodies
    UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
    Binding Specificity
    • 2
    • 2
    • 1
    AA 3-317
    Reactivity
    Human, Mouse, Rat, Zebrafish (Danio rerio)
    Host
    • 3
    • 3
    • 1
    • 1
    Rabbit
    Clonality
    • 7
    • 1
    Polyclonal
    Conjugate
    • 8
    This UNC13A/Munc13-1 antibody is un-conjugated
    Application
    • 8
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Specificity
    Specific for munc 13-1.
    Purification
    antiserum
    Immunogen
    Recombinant rat munc 13-1 (aa 3-317).
  • Application Notes
    WB: 1 : 1000 (AP staining)
    ICC: 1 : 500
    IHC: 1 : 200
    ELISA: not tested yet
    Comment

    IP: For most effective IP use the solubilization protocol described in the ELISA protocol. Consider that protein-protein interaction may be affected. ICC: This antibody gives much better results in ICC than the monoclonal antibody. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742276 as capture antibody (protocol for sandwich-ELISA).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
    Buffer
    PBS
    Handling Advice
    Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
    Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.
    Storage
    4 °C/-20 °C
    Storage Comment
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Fernandes, Riordan, Nomura, Remmers, Kraniotis, Marshall, Kukreja, Vassar, Contractor: "Epac2 Mediates cAMP-Dependent Potentiation of Neurotransmission in the Hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 16, pp. 6544-53, (2015) (PubMed).

    Juranek, Mukherjee, Siddiqui, Kaplan, Li, Ahnert-Hilger, Jahn, Calka: "Active zone protein expression changes at the key stages of cerebellar cortex neurogenesis in the rat." in: Acta histochemica, Vol. 115, Issue 6, pp. 616-25, (2013) (PubMed).

    Rinetti, Schweizer: "Ubiquitination acutely regulates presynaptic neurotransmitter release in mammalian neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 9, pp. 3157-66, (2010) (PubMed).

  • Target
    UNC13A/Munc13-1 (UNC13A) (Unc-13 Homolog A (UNC13A))
    Alternative Name
    Munc 13-1 (UNC13A Products)
    Synonyms
    Munc13-1 antibody, Unc13h1 antibody, 2410078G03Rik antibody, unc-13 homolog A antibody, unc-13 homolog A (C. elegans) antibody, Unc13a antibody, UNC13A antibody
    Pathways
    Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis
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