SPARCL1 antibody (C-Term)
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- Target See all SPARCL1 Antibodies
- SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))
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Binding Specificity
- AA 649-664, C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SPARCL1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for SPARC-like protein 1(SPARCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Sequence
- HCFGIKEEDI DENLLF
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for SPARC-like protein 1(SPARCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: SPARC-like 1(hevin)
Protein Name: SPARC-like protein 1 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1(649-664aa HCFGIKEEDIDENLLF), identical to the related mouse and rat sequences.
- Isotype
- IgG
- Top Product
- Discover our top product SPARCL1 Primary Antibody
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anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 208-236) antibody
SPARCL1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB24552 unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) antibodySPARCL1 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 17-300) antibodySPARCL1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 298-545) antibodySPARCL1 Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-SPARC-Like 1 (Hevin) (SPARCL1) (AA 215-244), (Middle Region) antibodySPARCL1 Reactivity: Human WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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- Target
- SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))
- Alternative Name
- SPARCL1 (SPARCL1 Products)
- Synonyms
- LOC445896 antibody, SPARCL1 antibody, sparcl antibody, sparcl1 antibody, DKFZp459I2327 antibody, Ecm2 antibody, Sc1 antibody, hevin antibody, mast9 antibody, PIG33 antibody, SC1 antibody, SPARCL-1 antibody, SPARCL1 antibody, SPARC like 1 antibody, SPARC-like 1 antibody, SPARC-like protein 1 antibody, LOC445896 antibody, SPARCL1 antibody, sparcl1 antibody, Sparcl1 antibody
- Background
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SPARCL1(SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules(HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration(Girard and Springer, 1995).
Synonyms: Ecm2 antibody|Extracellular matrix protein 2 antibody|Hevin antibody|High endothelial venule protein antibody|MAST 9 antibody|mast9 antibody|Matrix glycoprotein Sc1 antibody|PIG33 antibody|proliferation inducing protein 33 antibody|RAGS1 antigen antibody|SC1 antibody|SPARC like 1 antibody|SPARC-like protein 1 antibody|Sparcl1 antibody|SPRL1_HUMAN antibody - UniProt
- Q14515
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