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Sonic Hedgehog antibody (AA 21-120)

Name: Sonic Hedgehog antibody (AA 21-120)
SKU: ABIN731108
Price: 384.62 USD
Availability: InStock

SHH Reactivity: Human, Mouse, Rat, Pig WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse embryo lysates probed with Rabbit Anti-Shh Polyclonal Antibody, Unconjugated at 1:5000 for 90 min at 37˚C. (Sonic Hedgehog antibody (AA 21-120))

Formalin-fixed and paraffin embedded human gastric cancer labeled with Anti-SHH Polyclonal Antibody, Unconjugated (ABIN731108) followed by conjugation to the secondary antibody and DAB staining (Sonic Hedgehog antibody (AA 21-120))

IHC

Human A549 cells probed with Rabbit Anti-Shh Polyclonal Antibody, Unconjugated . (Sonic Hedgehog antibody (AA 21-120))

FACS

This image was generously submitted by Dr. (Sonic Hedgehog antibody (AA 21-120))

IF (p)

Lane 1: mouse embryo lysates Lane 2: rat brain lysates probed with Anti ShhPolyclonal Antibody, Unconjugated (ABIN731108) at 1:200 in 4 °C. (Sonic Hedgehog antibody (AA 21-120))

SDS

Mouse stomach lysates probed with Shh Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C. (Sonic Hedgehog antibody (AA 21-120))

6 images

(10 references)

Catalog No. ABIN731108

Datasheet as PDF

Target See all Sonic Hedgehog (SHH) Antibodies

Sonic Hedgehog (SHH)
Binding Specificity
14

11

7

6

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 21-120
Reactivity
60

53

39

16

9

7

6

5

3

2

2

1

1

1

1

1

1

1
Human, Mouse, Rat, Pig
Host
72

10

8

1

1
Rabbit
Compatible Secondaries
Clonality
65

27
Polyclonal
Conjugate
52

10

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This Sonic Hedgehog antibody is un-conjugated
Application
72

31

25

13

12

10

10

8

7

7

5

3

3

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Pig, Rat

Predicted Reactivity

Cow,Horse,Chicken,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Shh

Isotype

IgG
anti-Sonic Hedgehog (SHH) (AA 26-161) antibody
SHH Reactivity: Human, Mouse, Monkey WB, IHC, ELISA, FACS Host: Mouse Monoclonal 5H4 unconjugated

anti-Sonic Hedgehog (SHH) (C-Term) antibody
Verified SHH Reactivity: Human, Mouse WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (N-Term) antibody
SHH Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Guinea Pig, Horse, Goat, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 36-193) antibody
SHH Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 36-193) antibody
SHH Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D6 unconjugated

anti-Sonic Hedgehog (SHH) (AA 58-91), (N-Term) antibody
SHH Reactivity: Human, Mouse, Rat WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB52387 unconjugated

anti-Sonic Hedgehog (SHH) (AA 24-197) antibody
SHH Reactivity: Human, Rat, Dog, Monkey WB, IF Host: Mouse Monoclonal 10H6 unconjugated

anti-Sonic Hedgehog (SHH) (AA 237-399) antibody
SHH Reactivity: Rat WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 203-218), (N-Term) antibody
SHH Reactivity: Human, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 28-193) antibody
SHH Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Fan, Li, Liu, Cong, Zhao, Cao, Zheng: "Doxorubicin combined with low intensity ultrasound suppresses the growth of oral squamous cell carcinoma in culture and in xenografts." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 163, (2018) (PubMed).

Yang, Wu, Feng, Huang, Liu, Liu, Chen: "Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 267, (2017) (PubMed).

Song, Du, Tao: "Blockade of sonic hedgehog signaling decreases viability and induces apoptosis in retinoblastoma cells: The key role of the PI3K/Akt pathway." in: Oncology letters, Vol. 14, Issue 4, pp. 4099-4105, (2017) (PubMed).

Wang, Zhang, Bai, Wang, Du: "Baicalin Attenuates Alcoholic Liver Injury through Modulation of Hepatic Oxidative Stress, Inflammation and Sonic Hedgehog Pathway in Rats." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 3, pp. 1129-40, (2016) (PubMed).

Bai, Lu, Lin, Xu, Hu, Liang, Hong, Chen: "Sonic hedgehog-mediated epithelial-mesenchymal transition in renal tubulointerstitial fibrosis." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1317-27, (2016) (PubMed).

Wu, Zhao, Cheng, Yang, Huang, Meng, Wu, Zhang, Lv, Li: "Melittin induces PTCH1 expression by down-regulating MeCP2 in human hepatocellular carcinoma SMMC-7721 cells." in: Toxicology and applied pharmacology, Vol. 288, Issue 1, pp. 74-83, (2015) (PubMed).

Ma, Li, Li, Yang, Huang, Meng, Zhang, Li: "Geniposide alleviates inflammation by suppressing MeCP2 in mice with carbon tetrachloride-induced acute liver injury and LPS-treated THP-1 cells." in: International immunopharmacology, (2015) (PubMed).

Fan, Wang, Zhao, Liu, Chen, Sun, Zheng: "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 7, pp. 41, (2014) (PubMed).

Weiss, Guo, Shan, Shi, Romano, Sinha, Cantley, Guo: "Brg1 determines urothelial cell fate during ureter development." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 4, pp. 618-26, (2013) (PubMed).

Du, Feng, Wang, Piao, Cui, Chen, Lei, Sun, Liu, Wang, Li, Yang, Sun, Zhao, Jiang, Li, Jiang: "Curcumin suppresses malignant glioma cells growth and induces apoptosis by inhibition of SHH/GLI1 signaling pathway in vitro and vivo." in: CNS neuroscience & therapeutics, Vol. 19, Issue 12, pp. 926-36, (2013) (PubMed).
Target

Sonic Hedgehog (SHH)

Alternative Name

Shh (SHH Products)

Synonyms

HHG1 antibody, HLP3 antibody, HPE3 antibody, MCOPCB5 antibody, SMMCI antibody, TPT antibody, TPTPS antibody, 9530036O11Rik antibody, Dsh antibody, Hhg1 antibody, Hx antibody, Hxl3 antibody, M100081 antibody, fc83d08 antibody, shh antibody, syu antibody, vhh-1 antibody, vhh1 antibody, wu:fc83d08 antibody, Xhh antibody, hedgehog antibody, xshh antibody, SHH antibody, twh antibody, twhh antibody, sonic hedgehog antibody, sonic hedgehog a antibody, sonic hedgehog L homeolog antibody, sonic hedgehog protein A antibody, sonic hedgehog b antibody, SHH antibody, Shh antibody, shha antibody, shh.L antibody, shh antibody, shhb antibody

Background

Synonyms: TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5, Sonic hedgehog protein, SHH, HHG-1
Background: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

Gene ID

6469

UniProt

Q15465

Pathways

Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development