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GAPDH antibody (C-Term)

Name: GAPDH antibody (C-Term)
Brand: antibodies-online
SKU: ABIN1440016
Price: 718.74 USD
Availability: InStock

GAPDH Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Monkey, Dog, Cow, Sheep, Hamster, Horse, Goat, Guinea Pig, Cat, Fish, Avian, Donkey WB, IF, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated

Western Blotting (WB) image for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody (ABIN1440016)

Endogenous ERP57- endoplasmic reticulum lumen marker detected With AB0004 at 1/500 dilunon, lysates at 100 µg per Iane and rabbit polyclonal to goat lµg (HRP) at 1/10,000 dilution,

4 images

(8 references)

Catalog No. ABIN1440016

Datasheet as PDF

Target See all GAPDH Antibodies

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Binding Specificity
36

21

13

9

7

6

6

5

4

4

3

3

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
211

141

134

74

64

54

35

27

22

20

19

19

18

16

12

11

10

9

8

7

6

4

4

4

4

3

3

3

3

2

2

1

1

1

1

1

1
Human, Mouse, Rat, Rabbit, Pig, Chicken, Monkey, Dog, Cow, Sheep, Hamster, Horse, Goat, Guinea Pig, Cat, Fish, Avian, Donkey
Host
159

97

16

10

4
Goat
Compatible Secondaries
Clonality
179

105
Polyclonal
Conjugate
155

35

22

16

5

5

5

5

4

4

4

3

3

3

3

3

2

2

2

1

1

1

1

1
This GAPDH antibody is un-conjugated
Application
233

98

72

70

46

40

35

30

16

16

15

14

8

6

5

4

4

3

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

Detects a band of 37 kDa by Western blot in the following human (293A, HMEC-1, U-118, HaCat), rat (TR-iBRB), mouse (AtT-20, Hepa), canine (D17) and monkey (COS-7) whole cell lysates.

Characteristics

Glyceraldehyde-3-phosphate dehydrogenase

Purification

epitope affinity purified

Immunogen

Purified recombinant peptide derived from within residues 240 aa to the C-terminus of human GAPDH produced in E. coli.

Isotype

IgG

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GAPDH Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB16537 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibody
GAPDH Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibody
Verified GAPDH Reactivity: Human, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

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GAPDH Reactivity: Human WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB16543 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibody
Verified GAPDH Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibody
GAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated

anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibody
GAPDH Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

Restrictions

For Research Use only
Concentration

2 mg/mL

Buffer

Polyclonal antibody supplied as a 200 µL (2 mg/mL) aliquot in PBS, 20 % glycerol and 0.05 % sodium azide

Handling Advice

The antibody solution should be gently mixed before use.

Storage

4 °C,-20 °C

Storage Comment

For continuous use, store at 2-8 deg,C for one-two days. For extended storage, store in -20 deg,C freezer. Working dilution samples should be discarded if not used within 12 hours.
Ferreira, Rosa Soares, Ramalho, Ribeiro-Rodrigues, Máximo, Zuzarte, Girão, Pereira: "Exosomes and STUB1/CHIP cooperate to maintain intracellular proteostasis." in: PLoS ONE, Vol. 14, Issue 10, pp. e0223790, (2020) (PubMed).

Sanzà, Evans, Briggs, Cantero, Montoliu, Patel, Sviderskaya, Itzen, Figueiredo, Seabra, Hume: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).

Aires, Boia, Rodrigues-Neves, Madeira, Marques, Ambrósio, Santiago: "Blockade of microglial adenosine A2A receptor suppresses elevated pressure-induced inflammation, oxidative stress, and cell death in retinal cells." in: Glia, Vol. 67, Issue 5, pp. 896-914, (2019) (PubMed).

Robinson, Evans, Briggs, Ramalho, Hume: "Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport." in: Journal of cell science, Vol. 130, Issue 12, pp. 2056-2065, (2018) (PubMed).

Ramalho, Toscano, Pereira, Girão, Gonçalves, Marques: "Hyperglycemia-induced degradation of HIF-1α contributes to impaired response of cardiomyocytes to hypoxia." in: Revista portuguesa de cardiologia : orgão oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology, Vol. 36, Issue 5, pp. 367-373, (2018) (PubMed).

Ferreira, Soares, Ramalho, Pereira, Girao: "K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy." in: Scientific reports, Vol. 5, pp. 10210, (2016) (PubMed).

Casalou, Seixas, Portelinha, Pintado, Barros, Ramalho, Lopes, Barral: "Arl13b and the non-muscle myosin heavy chain IIA are required for circular dorsal ruffle formation and cell migration." in: Journal of cell science, Vol. 127, Issue Pt 12, pp. 2709-22, (2015) (PubMed).

Ribeiro-Rodrigues, Catarino, Marques, Ferreira, Martins-Marques, Pereira, Girão: "AMSH-mediated deubiquitination of Cx43 regulates internalization and degradation of gap junctions." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 11, pp. 4629-41, (2015) (PubMed).
Target

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name

GAPDH (GAPDH Products)

Synonyms

G3PD antibody, GAPD antibody, Gapd antibody, BEST:GH12586 antibody, CG12055 antibody, Dmel\\CG12055 antibody, GA3PDH antibody, GADPH antibody, GAP antibody, GAPDH antibody, GAPDH I antibody, GAPDH-1 antibody, GAPDH1 antibody, GAPDHI antibody, Gapdh antibody, Gapdh-1 antibody, Gapdh43E antibody, gadph antibody, gapdh antibody, gapdh-1 antibody, gh12586 antibody, cb609 antibody, gapd antibody, mg:bb02e05 antibody, wu:fb33a10 antibody, wu:ft80f05 antibody, KNC-NDS6 antibody, g3pd antibody, G3PDH antibody, glyceraldehyde-3-phosphate dehydrogenase antibody, Glyceraldehyde 3 phosphate dehydrogenase 1 antibody, glyceraldehyde-3-phosphate dehydrogenase S homeolog antibody, glyceraldehyde-3-phosphate dehydrogenase, type I antibody, olfactory receptor 8K3 antibody, GAPDH antibody, Gapdh antibody, Gapdh1 antibody, gapdh antibody, gapdh.S antibody, gapDH antibody, LOC100404960 antibody, LOC614985 antibody

Background

GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains.
Synonyms: glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HGNC:4141, GAPDH antibody.
Ensembl identifier: ENSG00000111640