Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B. Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.
Cross-Reactivity (Details)
Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody reacts with human Anti-V-ATPase subunit B. Sequence homology predicts that it will also react with mouse, rat, chicken, bovine, and fruit fly V-ATPase subunit B 2.
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.03-0.125 µg/mL Western blot: 1.0 µg/mL Other applications: user-optimized
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid repeated freezing and thawing cycles.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.
Ahmad, Khundmiri, Pribble, Merchant, Ameen, Klein, Levi, Lederer: "Role of vacuolar ATPase in the trafficking of renal type IIa sodium-phosphate cotransporter." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 27, Issue 6, pp. 703-14, (2011) (PubMed).
The V-ATPase subunit B 2 is related to a gene that encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.Rabbit Anti-V-ATPase Subunit B 2 Polyclonal Antibody is developed in rabbit using a synthetic peptide conjugated to KLH. It is purified from rabbit antiserum by immunoaffinity chromatography and supplied as 40 ug aliquot at concentration of 0.5 mg/ml.