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PRAS40 antibody (pThr246)

Name: PRAS40 antibody (pThr246)
SKU: ABIN744953
Price: 384.62 USD
Availability: InStock

AKT1S1 Reactivity: Human WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human liver carcinoma labeled with Anti-Phospho-PRAS40 (Thr246) Polyclonal Antibody, Unconjugated (ABIN744953) at 1:200 followed by conjugation to the secondary antibody and DAB staining (PRAS40 antibody (pThr246))

IHC

ELISA

2 images

Catalog No. ABIN744953

Datasheet as PDF

Target See all PRAS40 (AKT1S1) Antibodies

PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))
Binding Specificity
15

10

8

7

7

6

5

5

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr246
Reactivity
87

36

35

7

5

5

4

4

1

1

1

1

1
Human
Host
79

9
Rabbit
Compatible Secondaries
Clonality
76

12
Polyclonal
Conjugate
39

8

7

5

3

3

3

3

3

3

3

3

1

1

1

1

1
This PRAS40 antibody is un-conjugated
Application
68

41

29

13

13

11

8

3

3

2

2
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

This phosphorylation site is homologous to Thr247 in Mouse and Rat.

Predicted Reactivity

Human,Mouse,Rat,Dog,Cow,Horse

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human PRAS40 around the phosphorylation site of Thr246

Isotype

IgG

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Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name

PRAS40 (AKT1S1 Products)

Synonyms

MGC81452 antibody, lobe antibody, pras40 antibody, fb34a04 antibody, wu:fb34a04 antibody, Lobe antibody, PRAS40 antibody, 1110012J22Rik antibody, AI227026 antibody, Lobel antibody, AKT1 substrate 1 (proline rich) S homeolog antibody, AKT1 substrate 1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 (proline-rich) antibody, akt1s1.S antibody, AKT1S1 antibody, akt1s1 antibody, Akt1s1 antibody

Background

Synonyms: Lobe, PRAS40, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1S1
Background: Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.

Gene ID

84335

UniProt

Q96B36

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect