Actin, gamma 1 antibody (N-Term)
(3 references)-
- Target See all Actin, gamma 1 (ACTG1) Antibodies
- Actin, gamma 1 (ACTG1)
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Actin, gamma 1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Cat, Dog, Human, Mouse, Rat
- Characteristics
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Rabbit Polyclonal antibody to alpha Sarcomeric Actin (actin, cytoplasmic 2)
alpha Sarcomeric Actin antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.
- Isotype
- IgG
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anti-Actin, gamma 1 (ACTG1) (AA 1-375) antibody
ACTG1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Actin, gamma 1 (ACTG1) (AA 188-215) antibodyACTG1 Reactivity: Human WB, ELISA, FACS, IF Host: Rabbit Polyclonal unconjugated
anti-Actin, gamma 1 (ACTG1) (AA 188-215) antibodyACTG1 Reactivity: Human WB, FACS, IF Host: Rabbit Polyclonal RB19691 unconjugated
anti-Actin, gamma 1 (ACTG1) (AA 1-119) antibodyACTG1 Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Actin, gamma 1 (ACTG1) (AA 13-166) antibodyACTG1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Actin, gamma 1 (ACTG1) (AA 1-375) antibodyACTG1 Reactivity: Human ELISA, PLA Host: Mouse Monoclonal 1G6 unconjugated
anti-Actin, gamma 1 (ACTG1) (N-Term) antibodyACTG1 Reactivity: Human, Mouse WB, IHC, IHC (p), IF Host: Mouse Monoclonal 2A3 unconjugated
anti-Actin, gamma 1 (ACTG1) (N-Term) antibodyACTG1 Reactivity: Human WB, ELISA, ICC, FACS, IHC (p), IHC (fro) Host: Mouse Monoclonal 2A3 unconjugated
anti-Actin, gamma 1 (ACTG1) antibodyACTG1 Reactivity: Human WB, IF/ICC, FACS Host: Rabbit Polyclonal unconjugated
anti-Actin, gamma 1 (ACTG1) antibodyACTG1 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- 1XPBS ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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: "Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions." in: Analytical biochemistry, Vol. 577, pp. 1-13, (2020) (PubMed).
: "Maintenance BEZ235 Treatment Prolongs the Therapeutic Effect of the Combination of BEZ235 and Radiotherapy for Colorectal Cancer." in: Cancers, Vol. 11, Issue 8, (2019) (PubMed).
: "Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes." in: Journal of diabetes research, Vol. 2018, pp. 8743874, (2018) (PubMed).
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: "Quantitative in situ proximity ligation assays examining protein interactions and phosphorylation during smooth muscle contractions." in: Analytical biochemistry, Vol. 577, pp. 1-13, (2020) (PubMed).
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- Target
- Actin, gamma 1 (ACTG1)
- Alternative Name
- actin gamma 1 (ACTG1 Products)
- Synonyms
- ACT antibody, ACTG antibody, BRWS2 antibody, DFNA20 antibody, DFNA26 antibody, ACTG1 antibody, ACTA1 antibody, actg1a antibody, Actg antibody, actg1b antibody, AL023024 antibody, Actl antibody, E51 antibody, actin antibody, cytoplasmic antibody, actin gamma 1 antibody, actin, gamma 1 a antibody, actin, gamma 1 antibody, actin gamma 1 S homeolog antibody, actin, gamma, cytoplasmic 1 antibody, Actin, gamma 1 antibody, ACTG1 antibody, actg1 antibody, Actg1 antibody, actg1.S antibody
- Background
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Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
Cellular Localization: Cytoplasm , cytoskeleton - Molecular Weight
- 42 kDa
- Gene ID
- 71
- UniProt
- P63261
- Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
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