Actin, gamma 1 antibody (AA 1-375)

Catalog No. ABIN7437917

Product Details anti-Actin, gamma 1 Antibody

Target: Actin, gamma 1 (ACTG1)

Binding Specificity: AA 1-375

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin, gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Gamma Actin (ACTG)

Specificity: The antibody is a rabbit polyclonal antibody raised against ACTG. It has been selected for its ability to recognize ACTG in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Gamma Actin (ACTG) corresdonding to Met1~Phe375 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Actin, gamma 1

Target: Actin, gamma 1 (ACTG1)

Alternative Name: Gamma Actin (ACTG1 Products)

Synonyms: ACT antibody, ACTG antibody, BRWS2 antibody, DFNA20 antibody, DFNA26 antibody, ACTG1 antibody, ACTA1 antibody, actg1a antibody, Actg antibody, actg1b antibody, AL023024 antibody, Actl antibody, E51 antibody, actin antibody, cytoplasmic antibody, actin gamma 1 antibody, actin, gamma 1 a antibody, actin, gamma 1 antibody, actin gamma 1 S homeolog antibody, actin, gamma, cytoplasmic 1 antibody, Actin, gamma 1 antibody, ACTG1 antibody, actg1 antibody, Actg1 antibody, actg1.S antibody

Background: ACT, ACTB, ACTg1, DFNA20, DFNA26, deafness, autosomal dominant 20, deafness, autosomal dominant 26, Actin, cytoplasmic 2

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization