Calpastatin antibody
-
- Target See all Calpastatin (CAST) Antibodies
- Calpastatin (CAST)
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Calpastatin antibody is un-conjugated
-
Application
- ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant full-length human CAST protein
- Clone
- CAST-1550
- Isotype
- IgG1 kappa
-
anti-Calpastatin (CAST) (C-Term) antibody
CAST Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-667) antibodyCAST Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 582-669) antibodyCAST Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 2C5 unconjugated
anti-Calpastatin (CAST) (AA 275-310) antibodyCAST Reactivity: Human WB, IHC (p), FACS Host: Rabbit Polyclonal unconjugated
anti-Calpastatin (CAST) antibodyCAST Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 431-530) antibodyCAST Reactivity: Human, Mouse WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-686) antibodyCAST Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-667) antibodyCAST Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-Calpastatin (CAST) antibody (FITC)CAST Reactivity: Human WB, ELISA, IHC, IHC (p) Host: Rabbit Polyclonal FITC
anti-Calpastatin (CAST) antibodyCAST Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated
-
- Application Notes
-
Positive Control: HeLa cells. Esophagus or Small Intestine.
Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- Calpastatin (CAST)
- Alternative Name
- CAST (CAST Products)
- Synonyms
- BS-17 antibody, calp3 antibody, CAST antibody, sb:cb966 antibody, zgc:194249 antibody, cast antibody, DKFZp469E0716 antibody, calpastatin antibody, calpastatin S homeolog antibody, Calpastatin antibody, CAST antibody, Cast antibody, cast.S antibody, cast antibody, VDBG_09182 antibody, ical antibody
- Background
- Calpastatin is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpastatin, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpastatin co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.
- Molecular Weight
- 126kDa
- Gene ID
- 831
- UniProt
- P20810
-
