MAML2 antibody
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- Target See all MAML2 Antibodies
- MAML2 (Mastermind-Like 2 (MAML2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MAML2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human MAML2 fragment
- Clone
- MAML2-1302
- Isotype
- IgG2a kappa
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anti-Mastermind-Like 2 (MAML2) (AA 347-506) antibody
MAML2 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 796-894) antibodyMAML2 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 4A1 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 780-814) antibodyMAML2 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB52651 unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 750-850) antibodyMAML2 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 169-218) antibodyMAML2 Reactivity: Mouse WB Host: Rabbit Polyclonal unconjugated
anti-Mastermind-Like 2 (MAML2) (AA 324-373) antibodyMAML2 Reactivity: Human, Mouse WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Positive Control: MCF-7 cells. Pancreas or Placenta. Bladder or Colon Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- MAML2 (Mastermind-Like 2 (MAML2))
- Alternative Name
- MAML2 (MAML2 Products)
- Synonyms
- 5930431H10 antibody, BC032967 antibody, MAM-3 antibody, MAM2 antibody, MAM3 antibody, MLL-MAML2 antibody, mastermind like 2 (Drosophila) antibody, mastermind-like transcriptional coactivator 2 antibody, mastermind like transcriptional coactivator 2 antibody, Maml2 antibody, MAML2 antibody
- Background
- MAML2 (mastermind-like protein 2), also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML2 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-J a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene encoding MAML2 is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.
- Molecular Weight
- 125kDa
- Gene ID
- 84441
- UniProt
- Q8IZL2
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