ITM2B antibody (Internal Region)
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- Target See all ITM2B Antibodies
- ITM2B (Integral Membrane Protein 2B (ITM2B))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This ITM2B antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Purpose
- ITM2B
- Sequence
- HDKETYKLQR RETIK
- Cross-Reactivity
- Cow, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.
- Isotype
- IgG
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anti-Integral Membrane Protein 2B (ITM2B) (AA 81-148) antibody
ITM2B Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 218-247), (C-Term) antibodyITM2B Reactivity: Mouse WB, IHC (p) Host: Rabbit Polyclonal RB32712 unconjugated
anti-Integral Membrane Protein 2B (ITM2B) antibodyITM2B Reactivity: Human, Mouse, Rat ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 1-266) antibodyITM2B Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 1-266) antibodyITM2B Reactivity: Human WB Host: Mouse Polyclonal unconjugated
anti-Integral Membrane Protein 2B (ITM2B) (AA 146-245) antibodyITM2B Reactivity: Human ELISA, WB Host: Mouse Monoclonal 1A10 unconjugated
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2.5 μg/mL.
Western Blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat an
Peptide ELISA: antibody detection limit dilution 1:8000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomme
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- ITM2B (Integral Membrane Protein 2B (ITM2B))
- Alternative Name
- ITM2B (ITM2B Products)
- Synonyms
- ABRI antibody, BRI antibody, BRI2 antibody, BRICD2B antibody, E25B antibody, E3-16 antibody, FBD antibody, imBRI2 antibody, AI256040 antibody, Bri antibody, Bri2 antibody, Bricd2b antibody, D14Sel6 antibody, E25BMM antibody, itm2b antibody, zgc:55770 antibody, zgc:85688 antibody, wu:fc05f02 antibody, MGC52745 antibody, MGC76131 antibody, ITM2B antibody, itm2bl antibody, wu:fb94b03 antibody, zgc:64142 antibody, integral membrane protein 2B antibody, integral membrane protein 2Ba antibody, integral membrane protein 2B L homeolog antibody, integral membrane protein 2b antibody, integral membrane protein 2Bb antibody, ITM2B antibody, Itm2b antibody, itm2ba antibody, itm2b.L antibody, itm2b antibody, itm2bb antibody
- Background
- ITM2B, integral membrane protein 2B, ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, BRICHOS domain containing 2B, OTTHUMP00000018394
- Gene ID
- 9445, 16432, 290364
- NCBI Accession
- NP_068839
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