anti-Human PRKACA antibody for Immunofluorescence

Recommended PRKACA Antibody (supplied by: Log in to see )

Antigen
Protein Kinase A, alpha (PRKACA) Antibodies
  • PKCD
  • Pkaca
  • PKACA
  • PKA
  • Calpha
  • Cs-PKA
  • PKCA1
  • PRKAR1
  • protein kinase, cAMP dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, regulatory, type I, beta
  • Prkaca
  • PRKACA
  • PRKAR1B
Epitope
Center
20
16
12
7
7
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
111
29
24
4
3
2
2
2
2
2
2
Host
Rabbit
62
43
6
1
Clonality
Polyclonal
Conjugate
This PRKACA antibody is un-conjugated
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
95
42
15
13
11
10
5
4
3
2
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2855997
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.14905 ABIN2856513 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
12.14905 ABIN5078848 ICC IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN408069 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
1 ABIN317665 IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN1885909 IF WB Rabbit AA 1-340 Log in to see Polyclonal
1 ABIN5586148 IF IHC (p) WB Rabbit pThr197 Log in to see Polyclonal
1 ABIN5586147 ELISA IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2340986 IF IHC ELISA WB Mouse IgG1 kappa AA 563-672 Log in to see 2F11
1 ABIN4345788 ELISA ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2800195 IF (cc) IF WB Rabbit IgG AA 1-351 Log in to see Polyclonal

General

Antigen Protein Kinase A, alpha (PRKACA) Antibodies
Epitope Center
(20), (16), (12), (7), (7), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(111), (29), (24), (4), (3), (2), (2), (2), (2), (2), (2)
Host Rabbit
(62), (43), (6), (1)
Clonality
Polyclonal
Conjugate This PRKACA antibody is un-conjugated
(4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(95), (42), (15), (13), (11), (10), (5), (4), (3), (2), (2), (1), (1)
Supplier Log in to see

Product Details anti-PRKACA Antibody

Target Details PRKACA Application Details Handling Images
Cross-Reactivity Rat (Rattus), Dog (Canine), Sheep (Ovine), Cow (Bovine)
Cross-Reactivity (Details) Rat (100 %), Dog (100 %), Sheep (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to PKA C alpha (protein kinase, cAMP-dependent, catalytic, alpha)
PKA C alpha antibody [N2C3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human PKA C alpha. The exact sequence is proprietary.
Isotype IgG

Target Details PRKACA

Product Details anti-PRKACA Antibody Application Details Handling Images back to top
Antigen
Alternative Name PKA C alpha (PRKACA Antibody Abstract)
Background CAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Cytoplasm (By similarity) , Nucleus (By similarity)
Molecular Weight 41 kDa
Gene ID 5566
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals

Application Details

Product Details anti-PRKACA Antibody Target Details PRKACA Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HeLa , Jurkat , NT2D1 , mouse brain

Restrictions For Research Use only

Handling

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Images back to top
Format Liquid
Concentration 0.38 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-PRKACA Antibody Target Details PRKACA Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) WB Image Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE antibody diluted at...
Western Blotting (WB) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) WB Image Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE antibody dil...
Immunohistochemistry (IHC) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) IHC-P Image Immunohistochemical analysis of paraffin-embedded human colon, using PKA ...
Immunofluorescence (IF) image for anti-Protein Kinase A, alpha (PRKACA) (Center) antibody (ABIN2855997) ICC/IF Image Immunofluorescence analysis of methanol-fixed HeLa, using PKA alpha, ant...