Rabbit IgG Isotype Control

Details for Product No. ABIN1881001, Supplier: Log in to see
Isotype
Host
Rabbit
218
209
209
172
162
130
114
94
94
93
92
87
78
76
44
27
25
23
22
11
8
6
4
3
Clonality
Polyclonal
Application
ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Isotype IgG
Purification Purified by Protein A.
Background

Isotype controls are negative controls that are designed to measure the amount of non-specific background signal caused by the primary antibody. Isotype Control have no specificity for the target protein, yet retain all of the non-specific characteristics of an antibody.

Molecular Weight 150 kDa
Research Area Immunology, Secondary Antibodies
Application Notes WB(Assay-dependent)
ELISA(Assay-dependent)
FCM(Assay-dependent)
IHC-P(Assay-dependent)
IHC-F(Assay-dependent)
IF(IHC-P)(Assay-dependent)
IF(IHC-F)(Assay-dependent)
IF(ICC)(Assay-dependent)
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Product cited in: Xia, Jiao: "Histone variant H3.3 orchestrates neural stem cell differentiation in the developing brain." in: Cell death and differentiation, Vol. 24, Issue 9, pp. 1548-1563, 2017 (PubMed).

Chen, Qu, Li, Ye, Zhang, Qin, Fan: "Effects of 1,25-dihydroxyvitamin D3 in an ovalbumin-induced allergic rhinitis model." in: International immunopharmacology, Vol. 47, pp. 182-189, 2017 (PubMed). Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Mouse (Murine)).

Christensen, Juhl, Persson, Charabi, Mortensen, Kiss, Lelkaitis, Rubek, von Buchwald, Kjær: "uPAR-targeted optical near-infrared (NIR) fluorescence imaging and PET for image-guided surgery in head and neck cancer: proof-of-concept in orthotopic xenograft model." in: Oncotarget, Vol. 8, Issue 9, pp. 15407-15419, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Zhang, Zhao, Lin, Che, Li, Jiang, Hu, Wang: "Role of PTX3 in corneal epithelial innate immunity against Aspergillus fumigatus infection." in: Experimental eye research, 2016 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Yang, Jiang: "Embedding Raman Tags between Au Nanostar@Nanoshell for Multiplex Immunosensing." in: Small (Weinheim an der Bergstrasse, Germany), Vol. 12, Issue 36, pp. 4980-4985, 2016 (PubMed).

Brestoff, Kim, Saenz, Stine, Monticelli, Sonnenberg, Thome, Farber, Lutfy, Seale, Artis: "Group 2 innate lymphoid cells promote beiging of white adipose tissue and limit obesity." in: Nature, 2015 (PubMed). (Sample species: Mouse (Murine)).

Benesch, Ko, Tang, Dewald, Lopez-Campistrous, Zhao, Lai, Curtis, Brindley, McMullen: "Autotaxin is an inflammatory mediator and therapeutic target in thyroid cancer." in: Endocrine-related cancer, Vol. 22, Issue 4, pp. 593-607, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Benesch, Tang, Dewald, Dong, Mackey, Hemmings, McMullen, Brindley: "Tumor-induced inflammation in mammary adipose tissue stimulates a vicious cycle of autotaxin expression and breast cancer progression." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 9, pp. 3990-4000, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Chenna Narendra, Chalise, Magnusson, Uppugunduri: "Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis." in: PLoS ONE, Vol. 10, Issue 10, pp. e0141863, 2015 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Shang, Fu, Cheng, Wang, Liu, Zhao, Gu: "Photonic Crystal Microbubbles as Suspension Barcodes." in: Journal of the American Chemical Society, Vol. 137, Issue 49, pp. 15533-9, 2015 (PubMed).

Lee, Bae, Choi, Park, Chun: "Molecular cloning and expression analysis of pig CD7." in: Veterinary research communications, Vol. 38, Issue 3, pp. 257-63, 2014 (PubMed). (Sample species: Pig (Porcine)).

Zhuang, Hu, Tan, Zhu, Liu, Liu, Yan, Lin: "Overexpression of Lewis y antigen promotes human epididymis protein 4-mediated invasion and metastasis of ovarian cancer cells." in: Biochimie, Vol. 105, pp. 91-8, 2014 (PubMed).

Zhou, Xu, Zhang, Feng, Zhang, Zou, Liao: "Natural IgG antibodies in normal rabbit serum are involved in killing of the ompP2 mutant of Haemophilus parasuis SC096 strain via the classical complement pathway." in: Veterinary journal (London, England : 1997), Vol. 196, Issue 1, pp. 111-3, 2013 (PubMed). (Sample species: Others).

Huang, He, Gan, Ma: "Solidified liquid layer model makes quartz crystal microbalance a convenient molecular ruler." in: Colloids and surfaces. B, Biointerfaces, Vol. 85, Issue 1, pp. 92-6, 2011 (PubMed).

Ma, He, Liu, Gan, Jin, Zhou: "Surface initiated polymerization from substrates of low initiator density and its applications in biosensors." in: ACS applied materials & interfaces, Vol. 2, Issue 11, pp. 3223-30, 2010 (PubMed).