Rat IgG Isotype Control

Details for Product No. ABIN5633246, Supplier: Log in to see
Isotype
Host
Rat
177
174
172
139
126
116
82
67
64
62
56
52
51
47
34
33
22
16
15
11
7
4
3
Application
ELISA, Western Blotting (WB)
Options
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Isotype IgG
Cross-Reactivity (Details) Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
Purification Rat IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Isotype
Application Notes

Application Note: Rat IgG is suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins.

Western Blot Dilution: User Optimized

ELISA Dilution: User Optimized

Restrictions For Research Use only
Format Lyophilized
Reconstitution

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration 10.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Rat IgG isotype control (ABIN5633246) SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane ...
SDS-PAGE (SDS) image for Rat IgG isotype control (ABIN5633246) SDS-PAGE of Rat IgG Whole Molecule . Lane M: 3 µL Opal Prestained Marker . Lane 1: Re...