Rat IgG2a, kappa Isotype Control

Details for Product No. ABIN2656418, Supplier: Log in to see
Isotype
Host
Rat
77
64
1
Clonality (Clone)
Monoclonal ()
Application
Blocking Reagent (BR), Flow Cytometry (FACS), Immunofluorescence (IF), Isotype Control (IsoC), Immunohistochemistry (IHC)
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Clone RTK2758
Isotype IgG2a kappa
Fragment Chain kappa
Characteristics The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Purification The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.
Alternative Name IgG2a kappa
Research Area Secondary Antibodies
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.
Background publications Ma, Jiang, Gao, Cheng, Li, Huang, Lin, Li, Peng, Cai, Shao, Zhu, Han, Qin, Xia, Qi: "Immunosuppressive effect of compound K on islet transplantation in an STZ-induced diabetic mouse model." in: Diabetes, Vol. 63, Issue 10, pp. 3458-69, 2014 (PubMed).

Seach, Ueno, Fletcher, Lowen, Mattesich, Engwerda, Scott, Ware, Chidgey, Gray, Boyd: "The lymphotoxin pathway regulates Aire-independent expression of ectopic genes and chemokines in thymic stromal cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 8, pp. 5384-92, 2008 (PubMed).

Nishimoto, Lee, Hong, Potter, Maeda-Yamamoto, Kinoshita, Kawakami, Mittler, Kwon, Ware, Croft, Kawakami: "Costimulation of mast cells by 4-1BB, a member of the tumor necrosis factor receptor superfamily, with the high-affinity IgE receptor." in: Blood, Vol. 106, Issue 13, pp. 4241-8, 2005 (PubMed).

Jiang, Lagenaur, Narayanan: "Integrin-associated protein is a ligand for the P84 neural adhesion molecule." in: The Journal of biological chemistry, Vol. 274, Issue 2, pp. 559-62, 1999 (PubMed).